Yi Yan, Liang Aihua, Zhao Yong, Li Chunying, Wang Hailin, Liu Ting, Cao Chunyu, Hao Ran, Gao Shuangrong, Hui Liangqiang
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(14):1865-9.
To investigate the substance basis and the mechanism of pseudoanaphylactoid reactions (PR) induced by Shuanghuanglian injection (SHLI).
(1)The study of PR and the substance basis of PR of SHLI: ICR mice were divided into different test groups, the mice were intravenously injected with solutions of different concentration of SHLI, baicalin, forsythin, caffeotannic acid, positive control Compound 48/80 and normal sodium. All test substances were mixed with 0.4% Evans blue. The reaction and vascular permeability of the ears were observed and measured 30 min after SHLI injection. (2) The study of mechanisms: Mice were pretreated with an oral administration of Astemizol, intraperitoneal injection of cyclophosphamide 75 mg x kg(-1) or Compound 48/80 4 mg x kg(-1), then mice were intravenously injected with SHLI. At last, vascular permeability of the ears in pretreated groups was compared with SHLI treatment alone group.
SHLI of 300 mg x kg(-1) and 600 mg x kg(-1) caused obvious vascular hyperpermeability, but baicalin, forsythin and caffeotannic didn't cause vascular hyperpermeability in the ears. The Astemizol can decrease the degree of SHLI-induced vascular hyperpermeability of the ears in the mice. After intraperitoneal injected with cyclophosphamide, there was a slight decrease in the degree of SHLI-induced vascular hyperpermeability, but there was no marked changes in the degree of the SHLI-induced vascular hyperpermeability after the mice were pretreated with Compound 48/80.
SHLI in clinic equivalent dose can cause vascular hyperpermeability. Baicalin, forsythin and caffeotannic may not result in the PR of SHLI. The mechanism of the PR maybe relate to that SHLI stimulates histamine release, the activation of leucocyte maybe take part in the SHLI-induced PR, too. Antihistamine drug can prevent the genesis of PR which induced by SHLI.
探讨双黄连注射液（SHLI）引起类过敏反应（PR）的物质基础及机制。
（1）SHLI的PR及PR物质基础研究：将ICR小鼠分为不同试验组，分别静脉注射不同浓度的SHLI、黄芩苷、连翘苷、咖啡酸鞣质、阳性对照Compound 48/80和生理盐水。所有受试物质均与0.4%伊文思蓝混合。注射SHLI 30分钟后观察并测量耳部的反应及血管通透性。（2）机制研究：小鼠口服阿司咪唑、腹腔注射环磷酰胺75mg·kg⁻¹或Compound 48/80 4mg·kg⁻¹进行预处理，然后静脉注射SHLI。最后将预处理组耳部的血管通透性与单独SHLI处理组进行比较。
300mg·kg⁻¹和600mg·kg⁻¹的SHLI可引起明显的血管通透性增高，但黄芩苷、连翘苷和咖啡酸鞣质未引起耳部血管通透性增高。阿司咪唑可降低小鼠耳部SHLI诱导的血管通透性增高程度。腹腔注射环磷酰胺后，SHLI诱导的血管通透性增高程度略有降低，但用Compound 48/80预处理小鼠后，SHLI诱导的血管通透性增高程度无明显变化。
临床等效剂量的SHLI可引起血管通透性增高。黄芩苷、连翘苷和咖啡酸鞣质可能不会导致SHLI的PR。PR的机制可能与SHLI刺激组胺释放有关，白细胞的激活可能也参与了SHLI诱导的PR。抗组胺药物可预防SHLI诱导的PR的发生。
