Jiang Shu, Yang Meihua, Duan Jin'ao, Tao Jinhua, Qian Dawei
Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China.
Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(14):1936-40.
To investigate the effect of patulin producing strains on the different Chinese medicinal materials and the toxin biosynthesis mechanism.
Microbiology and HPLC analytical methods were adopted in this paper.
It was showed that the materials rich in starch and other polysaccharides were easily polluted by the patulin producing strain. This strain grew well and produced more toxins under 25 degrees C, 95% moisture content and bulk package. And the effect of low illumination intensity on the strain growth and toxin biosynthesis was not notable. Sample stability, precision, repeatability and rate of recovery were studied. HPLC analytic method was established and it revealed that the test method was suitable.
The pollution of Chinese medicinal materials by toxin producing microbes will be effectively controlled through establishing the suitable storage methods. So the study on the growing characteristics and toxin biosynthesis mechanism of toxin producing strains will be an important practical significance for controlling the toxin pollution of herbal medicines and contribute to establish the evaluation system of Chinese medicine safety.
研究展青霉素产生菌对不同中药材的影响及毒素生物合成机制。
本文采用微生物学和高效液相色谱分析方法。
结果表明，富含淀粉和其他多糖的药材易被展青霉素产生菌污染。该菌株在25℃、95%湿度和散装包装条件下生长良好且产生更多毒素。低光照强度对菌株生长和毒素生物合成的影响不显著。研究了样品稳定性、精密度、重复性和回收率。建立了高效液相色谱分析方法，结果表明该测试方法适用。
通过建立合适的储存方法可有效控制产毒微生物对中药材的污染。因此，研究产毒菌株的生长特性和毒素生物合成机制对于控制中药材毒素污染具有重要的现实意义，并有助于建立中药安全性评价体系。
