Palade Elena Alina, Demeter Zoltán, Hornyák Akos, Nemes Csaba, Kisary János, Rusvai Miklós
Department of Pathology and Forensic Veterinary Medicine, Faculty of Veterinary Science, Szent István University, 1078 Budapest, István utca 2, Hungary.
Avian Dis. 2011 Sep;55(3):468-75. doi: 10.1637/9688-021711-ResNote.1.
Samples collected in 2008 and 2009, from 49 turkey flocks of 6 to 43 days in age and presenting clinical signs of enteric disease and high mortality, were tested by polymerase chain reaction and reverse transcription-polymerase chain reaction for the presence of viruses currently associated with enteric disease (ED) syndromes: astrovirus, reovirus, rotavirus, coronavirus, adenovirus, and parvovirus. Turkey astroviruses were found in 83.67% of the cases and turkey astrovirus 2 (TAst-2) in 26.53%. The investigations directly demonstrated the high prevalence of turkey parvovirus (TuPV) in 23 flocks (46.9%) experiencing signs of ED, making this pathogen the second most identified after astroviruses. Phylogenetic analysis on a 527 base pair-long region from the NS1 gene revealed two main clusters, a chicken parvovirus (ChPV) and a TuPV group, but also the presence of a divergent branch of tentatively named "TuPV-like ChPV" strains. The 23 Hungarian TuPV strains were separately positioned in two groups from the American origin sequences in the TuPV cluster. An Avail-based restriction fragment length polymorphism assay has also been developed for the quick differentiation of TuPV, ChPV, and divergent TuPV-like ChPV strains. As most detected enteric viruses have been directly demonstrated in healthy turkey flocks as well, the epidemiology of this disease complex remains unclear, suggesting that a certain combination of pathogens, environmental factors, or both are necessary for the development of clinical signs.
对2008年和2009年采集的样本进行了检测。这些样本来自49个6至43日龄的火鸡群，呈现出肠道疾病临床症状且死亡率高，通过聚合酶链反应和逆转录 - 聚合酶链反应检测当前与肠道疾病（ED）综合征相关的病毒：星状病毒、呼肠孤病毒、轮状病毒、冠状病毒、腺病毒和细小病毒。在83.67%的病例中发现了火鸡星状病毒，26.53%的病例中发现了火鸡星状病毒2（TAst - 2）。调查直接表明，在出现ED症状的23个鸡群（46.9%）中火鸡细小病毒（TuPV）的高流行率，使该病原体成为仅次于星状病毒后第二大被鉴定出的病原体。对NS1基因527个碱基对长区域的系统发育分析揭示了两个主要聚类，一个是鸡细小病毒（ChPV）和一个TuPV组，但也存在一个暂定为“类TuPV的ChPV”菌株的分歧分支。23株匈牙利TuPV菌株在TuPV聚类中与美国来源序列分别位于两组。还开发了一种基于Avail的限制性片段长度多态性分析方法，用于快速区分TuPV、ChPV和分歧的类TuPV的ChPV菌株。由于在健康火鸡群中也直接检测到了大多数肠道病毒，这种疾病复合体的流行病学仍不清楚，这表明病原体、环境因素或两者的某种组合对于临床症状的发展是必要的。
