开发一种非靶向代谢组学方法来研究多囊肾病大鼠模型中的尿液。
Development of a non-targeted metabolomics method to investigate urine in a rat model of polycystic kidney disease.
机构信息
School of Natural Sciences Exercise, Edith Cowan University, Australia.
出版信息
Nephrology (Carlton). 2012 Feb;17(2):104-10. doi: 10.1111/j.1440-1797.2011.01532.x.
AIM
The purpose of this research was to use metabolomics to investigate the cystic phenotype in the Lewis polycystic kidney rat.
METHODS
Spot urine samples were collected from four male Lewis control and five male Lewis polycystic kidney rats aged 5 weeks, before kidney function was significantly impaired. Metabolites were extracted from urine and analysed using gas chromatography-mass spectrometry. Principal component analysis was used to determine key metabolites contributing to the variance observed between sample groups.
RESULTS
With the development of a metabolomics method to analyse Lewis and Lewis polycystic kidney rat urine, 2-ketoglutaric acid, allantoin, uric acid and hippuric acid were identified as potential biomarkers of cystic disease in the rat model.
CONCLUSION
The findings of this study demonstrate the potential of metabolomics to further investigate kidney disease.
目的
本研究旨在利用代谢组学方法研究 Lewis 多囊肾病大鼠的囊状表型。
方法
从 5 周龄的 4 只雄性 Lewis 对照组和 5 只雄性 Lewis 多囊肾病大鼠中收集尿样，此时肾功能尚未明显受损。从尿液中提取代谢物，并使用气相色谱-质谱联用技术进行分析。主成分分析用于确定导致样品组之间观察到的差异的关键代谢物。
结果
通过建立一种用于分析 Lewis 大鼠和 Lewis 多囊肾病大鼠尿液的代谢组学方法，鉴定出 2-酮戊二酸、尿囊素、尿酸和马尿酸可能是该大鼠模型中囊性疾病的生物标志物。
结论
本研究结果表明代谢组学具有进一步研究肾脏疾病的潜力。
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