Istituto del CNR di Chimica Biomolecolare, Via P. Gaifami 18, 95126 Catania, Italy.
J Agric Food Chem. 2011 Dec 14;59(23):12453-9. doi: 10.1021/jf203096q. Epub 2011 Nov 7.
This study investigated the phenolic composition and antioxidant activities of aqueous infusions from wild-grown caper (Capparis spinosa L.) and sea fennel (Crithmum maritimum L.) from the Dalmatia region (Croatia) before and after their submission to an in vitro digestion process. HPLC/UV-vis-DAD/ESI-MS analysis of the caper infusion identified rutin, kaempferol 3-O-rutinoside, and isorhamnetin 3-O-rutinoside as dominant flavonoids in the matrix together with a series of cinnamoylquinic acid derivatives, whereas in the sea fennel aqueous infusion chlorogenic acid (5-caffeoylquinic acid), its isomers, and higher derivatives were identified as almost the sole class of phenolics. Both infusions exhibited good and dose-dependent antioxidant activity before in vitro digestion by the DPPH method, the β-carotene bleaching method, and copper-induced oxidation of human LDL. The amount of total phenolics (Folin-Ciocalteu assay) strongly decreased in digested samples (from 3.0 and 2.2% in caper and sea fennel infusions, respectively, to <1.0%), as did their antioxidant activity as measured by the three aforesaid methods. The results showed that the majority of phenolic compounds detected in both infusions are not stable under applied simulated gastrointestinal conditions and that the stability of these secondary metabolites strongly depends on the nature of the corresponding matrix.
本研究调查了在体外消化过程前后，来自达尔马提亚地区（克罗地亚）野生生长的刺山柑（Capparis spinosa L.）和海茴香（Crithmum maritimum L.）的水浸液的酚类成分和抗氧化活性。刺山柑水浸液的 HPLC/UV-vis-DAD/ESI-MS 分析鉴定出芦丁、山奈酚 3-O-芸香糖苷和异鼠李素 3-O-芸香糖苷为基质中的主要类黄酮，以及一系列肉桂酰奎宁酸衍生物，而在海茴香水浸液中，绿原酸（5-咖啡酰奎宁酸）及其异构体和更高的衍生物被鉴定为几乎唯一的酚类。在体外消化前，两种浸液均通过 DPPH 法、β-胡萝卜素漂白法和铜诱导人 LDL 氧化，表现出良好的、剂量依赖性的抗氧化活性。在消化样品中，总酚类物质的含量（福林-肖卡尔特法）大幅下降（分别从刺山柑和海茴香浸液中的 3.0%和 2.2%下降至<1.0%），三种方法测定的抗氧化活性也随之下降。结果表明，两种浸液中检测到的大多数酚类化合物在应用模拟胃肠道条件下不稳定，这些次生代谢物的稳定性强烈依赖于相应基质的性质。
