See W A, Taylor T O, Mack L A, Tartaglione T A, Opheim K E, Berger R E
Department of Urology, University of Washington, Seattle.
J Urol. 1990 Sep;144(3):780-4. doi: 10.1016/s0022-5347(17)39591-5.
A rat model of bacterial epididymitis was developed and characterized for use in assessing the impact of acute epididymal inflammation on antibiotic penetration into the epididymis. A 0.2 ml. intratesticular injection of a 0.5 McFarland standard suspension of E. coli resulted in histologically confirmed acute epididymitis in all animals studied. Inflammatory changes were detectable as early as 24 hours following inoculation and were progressive to the last assessment point at 11 days. Early testicular infarction was observed in association with epididymal inflammation. Serial transcrotal ultrasounds of infected animals showed progressive increase in epididymal size and a late decrease in testicular size. Serum and epididymal drug concentrations were assayed following a single dose of the antibiotic amdinocillin. Fifteen minutes following the peak serum level, the drug concentration in infected epididymis was 2.3-fold higher than the contralateral, non-infected epididymis. These data suggest that acute inflammation enhances antibiotic penetration into the infected epididymis. The model described provides a rapid, reproducible method to study epididymal drug delivery in normal and diseased states.
建立了细菌性附睾炎大鼠模型，并对其进行表征，以用于评估急性附睾炎症对抗生素渗入附睾的影响。向大鼠睾丸内注射0.2毫升0.5麦氏标准大肠杆菌悬液，在所有研究动物中均导致经组织学证实的急性附睾炎。接种后最早在24小时即可检测到炎症变化，且炎症变化持续发展至第11天的最后评估点。观察到早期睾丸梗死与附睾炎症相关。对受感染动物进行的系列经阴囊超声检查显示附睾大小逐渐增加，后期睾丸大小减小。在单剂量给予抗生素氨比西林后，测定血清和附睾中的药物浓度。在血清水平达到峰值后15分钟，感染侧附睾中的药物浓度比未感染的对侧附睾高2.3倍。这些数据表明急性炎症增强了抗生素渗入受感染附睾的能力。所描述的模型提供了一种快速、可重复的方法，用于研究正常和患病状态下附睾的药物递送情况。
