Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre CEP 90050-170, Rio Grande do Sul (RS), Brazil.
Vet Microbiol. 2012 Mar 23;155(2-4):230-6. doi: 10.1016/j.vetmic.2011.09.021. Epub 2011 Sep 25.
A genome of a virus preliminarily named avian gyrovirus 2 (AGV2), a close relative to chicken anemia virus, was recently discovered in a chicken in the state of Rio Grande do Sul, Southern Brazil. To study the occurrence of AGV2 in Rio Grande do Sul and the neighboring state Santa Catarina, a number of adult chickens (n=108 and n=48, respectively) were tested for the presence of AGV2 DNA. An AGV2-specific PCR was developed, optimized and used to analyze DNA extracted from clinical samples. AGV2 DNA was detected in 98/108 (90.7%) of samples collected in the state of Rio Grande do Sul and 29/48 (60.4%) of the samples collected in the state of Santa Catarina. In order to check whether AGV2 DNA would be detected in samples from a geographically distant region, DNA from brain samples of 21 diseased chickens from the Netherlands were tested independently, by the same method. In such specimens, 9/21 (42.9%) brain tissue samples were found to contain AVG2 DNA. Sequence analysis of some of the PCR products demonstrated that the amplified AGV2 sequences could vary up to 15.8% and could preliminarily be divided in three groups. This indicated the occurrence of variants of AGV2, which may reflect differences in geographical origin and/or in biological properties. The data presented here provides evidence that AGV2 seems fairly distributed in chickens in Southern Brazil and that AGV2 also circulates in the Netherlands. Besides, circulating viruses display genetic variants whose significance should be further examined, particularly to determine whether AGV2 would play any role in chicken diseases.
最近在巴西南里奥格兰德州的一只鸡中发现了一种初步命名为禽回转病毒 2（AGV2）的病毒基因组，它是鸡贫血病毒的近亲。为了研究 AGV2 在南里奥格兰德州和邻近的圣卡塔琳娜州的发生情况，对 108 只成年鸡（n=108 和 n=48）和 48 只成年鸡（n=48）进行了检测，以检测 AGV2 DNA 的存在。开发、优化了一种 AGV2 特异性 PCR，并用于分析从临床样本中提取的 DNA。在南里奥格兰德州采集的 108 份样本中，有 98/108（90.7%）份样本检测到 AGV2 DNA，在圣卡塔琳娜州采集的 48 份样本中，有 29/48（60.4%）份样本检测到 AGV2 DNA。为了检查 AGV2 DNA 是否会在来自地理上遥远地区的样本中被检测到，用同样的方法独立检测了来自荷兰的 21 只患病鸡的脑组织样本的 DNA。在这些标本中，有 9/21（42.9%）脑组织样本含有 AGV2 DNA。对一些 PCR 产物的序列分析表明，扩增的 AGV2 序列可变异高达 15.8%，并可初步分为三组。这表明 AGV2 发生了变异，这可能反映了地理起源和/或生物特性的差异。本研究提供的证据表明，AGV2 在巴西南里奥格兰德州的鸡中分布相当广泛，AGV2 也在荷兰流行。此外，循环病毒显示出遗传变异，其意义应进一步研究，特别是要确定 AGV2 是否会在鸡病中发挥任何作用。
