Varela Ana Paula Muterle, Dos Santos Helton Fernandes, Cibulski Samuel Paulo, Scheffer Camila Mengue, Schmidt Candice, Sales Lima Francisco Esmaile, Silva Alessandra D'Avila, Esteves Paulo Augusto, Franco Ana Cláudia, Roehe Paulo Michel
FEPAGRO - Saúde Animal, Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul CEP 92990-000, Rio Grande do Sul, Brazil; Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, UFRGS. Av. Sarmento Leite 500, Sala 208, Porto Alegre CEP 90050-170, Rio Grande do Sul, Brazil.
FEPAGRO - Saúde Animal, Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul CEP 92990-000, Rio Grande do Sul, Brazil.
Biologicals. 2014 Nov;42(6):346-50. doi: 10.1016/j.biologicals.2014.08.002. Epub 2014 Oct 27.
This study focuses on the detection of chicken anemia virus (CAV) and avian gyrovirus 2 (AGV2) genomes in commercially available poultry vaccines. A duplex quantitative real-time PCR (dqPCR), capable of identifying genomes of both viruses in a single assay, was employed to determine the viral loads of these agents in commercially available vaccines. Thirty five vaccines from eight manufacturers (32 prepared with live and 3 with inactivated microorganisms) were examined. Genomes of CAV were detected as contaminants in 6/32 live vaccines and in 1/3 inactivated vaccines. The CAV genome loads ranged from 6.4 to 173.4 per 50 ng of vaccine DNA (equivalent to 0.07 to 0.69 genome copies per dose of vaccine). Likewise, AGV2 genomes were detected in 9/32 live vaccines, with viral loads ranging from 93 to 156,187 per 50 ng of vaccine DNA (equivalent to 0.28-9176 genome copies per dose of vaccine). These findings provide evidence for the possibility of contamination of poultry vaccines with CAV and AGV2 and they also emphasize the need of searching for these agents in vaccines in order to ensure the absence of such potential contaminants.
本研究聚焦于检测市售家禽疫苗中鸡贫血病毒（CAV）和禽环病毒2型（AGV2）的基因组。采用一种能够在单一检测中鉴定两种病毒基因组的双重定量实时PCR（dqPCR）方法，来测定市售疫苗中这些病原体的病毒载量。对来自八个制造商的35种疫苗（32种由活微生物制备，3种由灭活微生物制备）进行了检测。在6/32种活疫苗和1/3种灭活疫苗中检测到CAV基因组作为污染物。CAV基因组载量为每50 ng疫苗DNA 6.4至173.4个（相当于每剂疫苗0.07至0.69个基因组拷贝）。同样，在9/32种活疫苗中检测到AGV2基因组，病毒载量为每50 ng疫苗DNA 93至156,187个（相当于每剂疫苗0.28至9176个基因组拷贝）。这些发现为家禽疫苗被CAV和AGV2污染的可能性提供了证据，同时也强调了在疫苗中查找这些病原体以确保不存在此类潜在污染物的必要性。
