Wauters Jella, Franck Thierry, Pille Frederik, Martens Ann, Demeyere Kristel, Sys Stanislas, Serteyn Didier, Gasthuys Frank, Meyer Evelyne
Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Salisburylane 133, 9820 Merelbeke, Belgium.
Vet Immunol Immunopathol. 2011 Dec 15;144(3-4):417-22. doi: 10.1016/j.vetimm.2011.09.009. Epub 2011 Oct 1.
Myeloperoxidase (MPO) is a protein of interest due to its involvement in equine pathologies. Until now, results in equine diagnostic research were achieved through extracellular MPO detection. However, studying the cellular MPO content in neutrophils has revealed important insights in human diseases. This study aimed to develop a technique for the specific detection of MPO on the single cell level defining a flow cytometric protocol for the detection of both equine surface-bound and cellular MPO. Both indirect and direct labeling techniques are described which include the comparison of two secondary antibodies and two linking-fluorochromes, respectively.
髓过氧化物酶（MPO）因其与马的病理学有关而备受关注。到目前为止，马诊断研究的结果是通过细胞外MPO检测获得的。然而，对中性粒细胞中细胞MPO含量的研究为人类疾病提供了重要的见解。本研究旨在开发一种在单细胞水平上特异性检测MPO的技术，确定一种流式细胞术方案，用于检测马的表面结合型和细胞型MPO。文中描述了间接和直接标记技术，分别包括两种二抗和两种连接荧光染料的比较。
