Nanotechnology Research Group, Chemical and Biochemical Processing Division, Central Institute for Research on Cotton Technology, Adenwala Road, Matunga, Mumbai 400 019, India.
Bioresour Technol. 2012 Jan;103(1):219-26. doi: 10.1016/j.biortech.2011.09.110. Epub 2011 Oct 5.
Fenton's reagent that generates reactive hydroxyl radical species was evaluated for its effectiveness as a pretreatment agent on cotton cellulosic substrates to increase its susceptibility to cellulase enzyme. Response surface methodology was used to optimize four different process variables viz., time of reaction; substrate size and concentrations of Fe2+ and H2O2. Overall, the cellulose substrates treated at 0.5 mM concentration of Fe2+, 2% concentration of H2O2 for a reaction period of 48 h gave the highest enzyme activity as determined using the response surface methodology. Cellulose substrates with high aspect ratio recorded better enzyme response than that with low aspect ratio which is supported by copper number estimation. The cellulosic substrate prepared using a combination of optimized Fenton's pretreatment conditions and/or enzyme hydrolysis were studied and characterized by atomic force microscopy and scanning electron microscopy. Additionally, degree of polymerization analysis gives further insight into the degradation during Fenton's reaction.
芬顿试剂可产生具有反应活性的羟自由基，本文评估了其作为预处理剂对棉纤维素底物的有效性，以提高其对纤维素酶的敏感性。采用响应面法优化了四个不同的工艺变量，即反应时间、底物尺寸以及 Fe2+和 H2O2 的浓度。总体而言，用 0.5 mM 的 Fe2+浓度、2%的 H2O2 浓度、48 小时的反应时间处理的纤维素底物，通过响应面法测定，表现出最高的酶活性。高纵横比的纤维素底物比低纵横比的底物具有更好的酶反应，这一结果得到了铜值估计的支持。使用优化的芬顿预处理条件和/或酶水解组合制备的纤维素底物，通过原子力显微镜和扫描电子显微镜进行了研究和表征。此外，聚合度分析进一步深入了解了芬顿反应过程中的降解情况。
