Department of Biological Engineering, Inha University, Incheon 402-751, South Korea.
Bioresour Technol. 2011 Jan;102(2):1214-9. doi: 10.1016/j.biortech.2010.07.108. Epub 2010 Aug 3.
For increasing cellulose accessibility to the enzymatic attack, the pretreatment is a necessary step to alter some structural characteristics of cellulosic materials. As a new pretreatment method, microwave irradiation on cellulose dissolution pretreatment with ionic liquids (ILs) was investigated in this study. Microwave irradiation not only enhanced the solubility of cellulose in ILs but also significantly decreased the degree of polymerization of regenerated cellulose after IL dissolution pretreatment, resulting in significant improvement of cellulose hydrolysis. The rate of enzymatic hydrolysis of cotton cellulose was increased by at least 12-fold after IL dissolution pretreatment at 110 °C and by 50-fold after IL dissolution pretreatment with microwave irradiation. Our results demonstrate that cellulose pretreatment with ILs and microwave irradiation is a potential alternative method for the pretreatment of cellulosic materials.
为了提高纤维素对酶攻击的可及性，预处理是改变纤维素材料某些结构特性的必要步骤。作为一种新的预处理方法，本研究考察了微波辐射对纤维素在离子液体（ILs）中的溶解预处理的影响。微波辐射不仅提高了纤维素在 ILs 中的溶解度，而且还显著降低了 IL 溶解预处理后再生纤维素的聚合度，从而显著提高了纤维素的水解。在 110°C 下进行 IL 溶解预处理后，棉纤维素的酶水解速率至少提高了 12 倍，而经过微波辐射的 IL 溶解预处理后，酶水解速率提高了 50 倍。我们的结果表明，纤维素用 ILs 和微波辐射预处理是一种有潜力的纤维素材料预处理替代方法。
