CICECO, Departamento de Química, Universidade de Aveiro, Campus Universitário de Santiago, P-3810-193 Aveiro, Portugal.
Bioresour Technol. 2012 Jan;103(1):131-5. doi: 10.1016/j.biortech.2011.09.095. Epub 2011 Oct 1.
The fermentation of reducing sugars from hardwood (eucalypt) spent sulphite liquor (HSSL) into ethanol by Pichia (Scheffersomyces) stipitis is hindered by concomitant inhibitors of microbial metabolism. The conditions for the HSSL biological treatment step by Paecilomyces variotii were evaluated and optimised. Two different strategies of reactor operation were compared using single batch (B) and sequential batch reactor (SBR). Biological treatment of HSSL in the SBR revealed the best results with respect to the removal of microbial inhibitors. Also, most of inhibitory compounds, acetic acid, gallic acid, pyrogallol, amongst others, were removed from HSSL by P. variotii before the ethanol fermentation. The bio-detoxified HSSL was subjected to a successful fermentation by P. stipitis, attaining a maximum ethanol concentration of 2.4 g L(-1) with a yield of 0.24 g ethanol g sugars(-1).
木质素（桉树）亚硫酸盐废液（HSSL）中的还原糖通过毕赤酵母（Scheffersomyces）发酵为乙醇的过程受到微生物代谢抑制剂的阻碍。评估并优化了多形拟青霉对 HSSL 进行生物处理的条件。通过单批（B）和序批式反应器（SBR）比较了两种不同的反应器操作策略。在 SBR 中对 HSSL 进行生物处理时，在去除微生物抑制剂方面取得了最佳效果。此外，多形拟青霉在对 HSSL 进行乙醇发酵之前，去除了大部分抑制化合物，如乙酸、没食子酸、焦儿茶酚等。经过生物解毒的 HSSL 成功地通过毕赤酵母发酵，达到了 2.4 g/L 的最大乙醇浓度，产率为 0.24 g 乙醇/g 糖。
