Departamento de Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, sala I-1-059, Av. Carlos Chagas Filho, 373 Cidade Universitária, CEP 21941-902, Rio de Janeiro, RJ, Brazil.
Res Microbiol. 2011 Dec;162(10):1052-9. doi: 10.1016/j.resmic.2011.10.002. Epub 2011 Oct 10.
Bacteriocins are peptides produced by bacteria and having inhibitory activity against other bacteria. Many of these substances may be useful as antibacterial agents for practical applications. In this study, 21 Staphylococcus spp. isolated from pigs, dogs and bovine milk in different states of Brazil were investigated for staphylococcin production. Hyicin 3682, a bacteriocin produced by one such strain, inhibited almost all strains tested, including Bacillus cereus, Listeria monocytogenes and Staphylococcus aureus. PCR experiments showed that hyicin 3682 is lantibiotic-related, but not identical, to both epidermin and Bsa. The maximum production of hyicin 3682 (6,400 AU/ml) was observed after 24 h of growth in BHI medium at 37 °C. Hyicin 3682 proved to be a cationic, small antimicrobial peptide with a molecular mass of 2,139 Da. It exhibited resistance to low pH and to heating at 65 °C, and partial sensitivity to proteolytic enzymes. Taken together, these results suggest that hyicin 3682, the first bacteriocin characterized in Staphylococcus hyicus, has potential biotechnological applications as a food preservative. Moreover, hyicin 3682 was able to inhibit its producer strain, suggesting that an effective immune system for specific protection against hyicin 3682 is not found in its producer strain, a characteristic not described thus far for other staphylococcins.
细菌素是由细菌产生的具有抑制其他细菌活性的肽。许多这些物质可能作为抗菌剂用于实际应用。在这项研究中，研究了从巴西不同州的猪、狗和牛乳中分离出的 21 株葡萄球菌属细菌，以研究其葡萄球菌素的产生情况。一种这样的菌株产生的抑菌素 3682 几乎可以抑制所有测试的菌株，包括蜡样芽孢杆菌、单核细胞增生李斯特菌和金黄色葡萄球菌。PCR 实验表明，抑菌素 3682 与表皮素和 Bsa 有关，但并不相同。在 37°C 下，在 BHI 培养基中生长 24 小时后，抑菌素 3682 的最大产量为 6400 AU/ml。抑菌素 3682 是一种阳离子、小的抗菌肽，分子量为 2139 Da。它对低 pH 和 65°C 的加热具有抗性，对蛋白酶具有部分敏感性。综上所述，这些结果表明，抑菌素 3682 是在猪葡萄球菌中首次被表征的细菌素，具有作为食品防腐剂的潜在生物技术应用。此外，抑菌素 3682 能够抑制其产生菌株，这表明在其产生菌株中没有发现针对抑菌素 3682 的有效免疫防御系统，这是迄今为止其他葡萄球菌素所没有描述的特征。
