转录和功能分析表明，钩藤碱通过抑制金黄色葡萄球菌自溶来发挥作用。
Transcriptional and functional analysis shows sodium houttuyfonate-mediated inhibition of autolysis in Staphylococcus aureus.
机构信息
Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
出版信息
Molecules. 2011 Oct 21;16(10):8848-65. doi: 10.3390/molecules16108848.
Sodium houttuyfonate (SH), an addition compound of sodium bisulfite and houttuynin, showed in vitro antibacterial activity against 21 Staphylococcus aureus (S. aureus) strains grown in planktonic cultures. Microarray results showed decreased levels of autolysin atl, sle1, cidA and lytN transcripts in the SH-treated strain as compared to the control strain, consistent with the induction of the autolytic repressors lrgAB and sarA and with the downregulation of the positive regulators agrA and RNAIII. Triton X-100-induced autolysis was significantly decreased by SH in S. aureus ATCC 25923, and quantitative bacteriolytic assays and zymographic analysis demonstrated SH-mediated reduction of extracellular murein hydrolase activity in these cells. Anti-biofilm assay showed that SH is poorly active against S. aureus grown in biofilm cultures, whereas SH diminished the amounts of extracellular DNA (eDNA) of S. aureus in a dose-dependent manner, which suggested that SH may impede biofilm formation by reducing the expression of cidA to inhibit autolysis and eDNA release in the early phase. Some of the microarray results were confirmed by real-time RT-PCR.
硫代硫酸氢钠（SH）是亚硫酸氢钠和鱼腥草素的加成化合物，对浮游培养的 21 株金黄色葡萄球菌（S. aureus）表现出体外抗菌活性。微阵列结果显示，与对照菌株相比，SH 处理的菌株中自溶酶 atl、sle1、cidA 和 lytN 转录本的水平降低，这与自溶抑制物 lrgAB 和 sarA 的诱导以及agrA 和 RNAIII 的下调一致。在金黄色葡萄球菌 ATCC 25923 中，Triton X-100 诱导的自溶作用显著被 SH 抑制，定量溶菌测定和酶谱分析表明，SH 介导了这些细胞中细胞外粘肽水解酶活性的降低。抗生物膜测定表明，SH 对生物膜培养物中生长的金黄色葡萄球菌活性较差，而 SH 以剂量依赖的方式减少金黄色葡萄球菌的细胞外 DNA（eDNA）量，这表明 SH 可能通过降低 cidA 的表达来抑制自溶和 eDNA 的早期释放，从而阻碍生物膜的形成。一些微阵列结果通过实时 RT-PCR 得到了证实。
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