Clinical Genetics Department, The Affiliated Shengjing Hospital, China Medical University, 110004 Shenyang, Liaoning, PR China.
Virus Res. 2012 Jan;163(1):223-8. doi: 10.1016/j.virusres.2011.09.034. Epub 2011 Oct 15.
The genome of human cytomegalovirus (HCMV) has been studied extensively, particularly in the UL/b' region. In this study, transcripts of one of the UL/b' genes, UL146, were identified in 3 HCMV isolates obtained from urine samples of congenitally infected infants.
Northern blot hybridization, cDNA library screening, and RACE-PCR were used.
All sequences of clones from a cDNA library were about 3225 bp in length with the same 5' and 3' ends. The results were accordant with that analyzed by Northen-blot and RACE-PCR in three HCMV clinical isolates. The transcript initiated from the region upstream of UL146 flanking region and terminated just downstream of UL132 including UL146, UL147, UL147A, UL148, and UL132 ORFs. Treatment of the infected cells with phosphonoacetic acid inhibited its transcription.
UL146 ORF was transcribed with 4 downstream ORFs from UL147 to UL132 at true late kinetics. The transcript of UL146 initiated at 73nt upstream of UL146 and terminated just downstream of UL132 in the 3 clinical isolates.
人类巨细胞病毒（HCMV）的基因组已被广泛研究，特别是在 UL/b' 区域。在这项研究中，从先天感染婴儿的尿液样本中获得的 3 个 HCMV 分离株中鉴定出 UL146 的 UL/b' 基因之一的转录本。
使用 Northern blot 杂交、cDNA 文库筛选和 RACE-PCR。
cDNA 文库中克隆的所有序列长度约为 3225bp，具有相同的 5'和 3'末端。这与 3 个 HCMV 临床分离株的 Northern blot 和 RACE-PCR 分析结果一致。该转录物从 UL146 侧翼区域上游的区域起始，并在包括 UL146、UL147、UL147A、UL148 和 UL132 ORFs 的 UL132 下游终止。用膦甲酸钠处理感染细胞可抑制其转录。
UL146 ORF 在真正的晚期动力学下与 UL147 到 UL132 的 4 个下游 ORF 转录。在 3 个临床分离株中，UL146 的转录物在 UL146 的上游 73nt 处起始，并在 UL132 的下游终止。
