China Medical University, Shenyang, Liaoning, China.
J Med Virol. 2011 Dec;83(12):2151-6. doi: 10.1002/jmv.22241.
The human cytomegalovirus UL145 gene is located between the highly variable genes UL144 and UL146 in the UL/b' region. However, unlike its neighboring genes, UL145 is relatively conserved among strains isolated from patients. The transcriptional features and transcript structure of UL145 has not yet been examined. In this study, the transcriptional features and structure of UL145 were characterized using RNA preparations from three HCMV strains isolated from patients, designated H, C, and X, respectively. Two transcripts were identified by cDNA library screening. Two main clusters of transcripts, one located between 500 and 700 nt, and the other between 1,400 and 1,700 nt, were confirmed by Northern blot analysis. Abundant transcripts were detected at 96 h post-infection by Northern blot, suggesting that UL145 was a late gene. The terminal sequences of transcripts obtained by 3' rapid amplification of cDNA ends demonstrated the presence of polyadenylation. Transcripts identified in the RNA of H, C, and X strains 96 h post-infection had the same 3' end but different 5' ends. In addition to polycistronic transcripts with upstream genes, UL145 could be transcribed as a single transcript during late strain infections.
人巨细胞病毒 UL145 基因位于 UL/b' 区域高度可变基因 UL144 和 UL146 之间。然而，与邻近基因不同的是，UL145 在从患者中分离的毒株中相对保守。UL145 的转录特征和转录结构尚未被检测到。在这项研究中，使用分别从三名患者中分离的三种 HCMV 株（分别命名为 H、C 和 X）的 RNA 制剂来表征 UL145 的转录特征和结构。通过 cDNA 文库筛选鉴定了两种转录本。通过 Northern blot 分析证实了两个主要的转录本簇，一个位于 500 到 700 nt 之间，另一个位于 1400 到 1700 nt 之间。通过 Northern blot 在感染后 96 小时检测到丰富的转录本，表明 UL145 是一个晚期基因。通过 3' 快速扩增 cDNA 末端获得的转录本末端序列表明存在多聚腺苷酸化。在感染后 96 小时的 H、C 和 X 株的 RNA 中鉴定出的转录本具有相同的 3' 端，但 5' 端不同。除了具有上游基因的多顺反子转录本外，UL145 在晚期感染的单一株中也可以作为单个转录本进行转录。
