人巨细胞病毒 UL144 转录本的特征。
Characterization of the transcripts of human cytomegalovirus UL144.
机构信息
Clinical Genetics Department, The Affiliated Shengjing Hospital, China Medical University, 110004 Shenyang, Liaoning of China.
出版信息
Virol J. 2011 Jun 14;8:299. doi: 10.1186/1743-422X-8-299.
BACKGROUND
The genome of human cytomegalovirus (HCMV) has been studied extensively, particularly in the UL/b' region. In this study, transcripts of one of the UL/b' genes, UL144, were identified in 3 HCMV isolates obtained from urine samples of congenitally infected infants.
METHODS
Northern blot hybridization, cDNA library screening, and RACE-PCR were used.
RESULTS
We identified at least 4 differentially regulated 3'-coterminal transcripts of UL144 in infected cells of 1,300, 1,600, 1,700, and 3,500 nucleotides (nt). The 1600 nt transcript was the major form of UL144 mRNA. The largest transcript initiated from the region within the UL141 open reading frame (ORF) and included UL141, UL142, UL143, UL144, and UL145 ORFs.
CONCLUSIONS
These findings reveal the complex nature of the transcription of the UL144 gene in clinical isolates.
背景
人类巨细胞病毒（HCMV）的基因组已被广泛研究，特别是在 UL/b' 区域。在这项研究中，从先天感染婴儿的尿液样本中获得的 3 株 HCMV 分离株中鉴定出 UL144 的 UL/b' 基因之一的转录本。
方法
使用 Northern blot 杂交、cDNA 文库筛选和 RACE-PCR。
结果
我们在感染细胞中鉴定出 UL144 的至少 4 种差异调节的 3'-末端转录本，大小分别为 1300、1600、1700 和 3500 个核苷酸（nt）。1600nt 转录本是 UL144 mRNA 的主要形式。最大的转录本起始于 UL141 开放阅读框（ORF）内的区域，并包含 UL141、UL142、UL143、UL144 和 UL145 ORFs。
结论
这些发现揭示了临床分离株中 UL144 基因转录的复杂性质。
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