用于鲍曼不动杆菌 ADP1 的表达载体。
Expression vectors for Acinetobacter baylyi ADP1.
机构信息
Emory University School of Medicine, Department of Biochemistry, Center for Fundamental and Applied Molecular Evolution, Atlanta, Georgia, USA.
出版信息
Appl Environ Microbiol. 2012 Jan;78(1):280-3. doi: 10.1128/AEM.05597-11. Epub 2011 Oct 21.
Abstract
Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous recombination, so it can be transformed without restriction digests or ligation reactions. Expression vectors for this system, however, are not yet widely available. Here we describe the construction and characterization of inducible expression vectors that replicate as plasmids in A. baylyi or integrate into a nonessential part of its chromosome. These tools will facilitate the engineering of genes and genomes in this promising model organism.
摘要
鲍曼不动杆菌 ADP1 天然具有感受态，并且擅长同源重组，因此无需限制酶消化或连接反应即可进行转化。然而，该系统的表达载体尚未广泛应用。在此，我们描述了诱导型表达载体的构建和特性，这些载体在鲍曼不动杆菌中作为质粒复制或整合到其染色体的非必需部分。这些工具将促进在这个有前途的模式生物中对基因和基因组进行工程改造。
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