Vaneechoutte Mario, Young David M, Ornston L Nicholas, De Baere Thierry, Nemec Alexandr, Van Der Reijden Tanny, Carr Emma, Tjernberg Ingela, Dijkshoorn Lenie
Department of Clinical Chemistry, Microbiology & Immunology, Ghent University Hospital, Blok A, De Pintelaan 185, 9000 Gent, Belgium.
Appl Environ Microbiol. 2006 Jan;72(1):932-6. doi: 10.1128/AEM.72.1.932-936.2006.
Genotypic and phenotypic analyses were carried out to clarify the taxonomic position of the naturally transformable Acinetobacter sp. strain ADP1. Transfer tDNA-PCR fingerprinting, 16S rRNA gene sequence analysis, and selective restriction fragment amplification (amplified fragment length polymorphism analysis) indicate that strain ADP1 and a second transformable strain, designated 93A2, are members of the newly described species Acinetobacter baylyi. Transformation assays demonstrate that the A. baylyi type strain B2(T) and two other originally identified members of the species (C5 and A7) also have the ability to undergo natural transformation at high frequencies, confirming that these five strains belong to a separate species of the genus Acinetobacter, characterized by the high transformability of its strains that have been cultured thus far.
进行了基因型和表型分析,以阐明可自然转化的不动杆菌属ADP1菌株的分类地位。转移tDNA-PCR指纹图谱、16S rRNA基因序列分析和选择性限制性片段扩增(扩增片段长度多态性分析)表明,ADP1菌株和另一株可转化菌株(命名为93A2)是新描述的拜氏不动杆菌物种的成员。转化试验表明,拜氏不动杆菌模式菌株B2(T)以及该物种最初鉴定的另外两个成员(C5和A7)也具有高频自然转化的能力,证实这五株菌株属于不动杆菌属的一个独立物种,其特征是迄今为止培养的菌株具有高转化能力。