Center for Fundamental and Applied Molecular Evolution, Department of Biochemistry, Emory University, Atlanta, Georgia, United States of America.
PLoS One. 2010 Oct 8;5(10):e13244. doi: 10.1371/journal.pone.0013244.
Most plasmids replicate only within a particular genus or family.
METHODOLOGY/PRINCIPAL FINDINGS: Here we describe an engineered high copy number expression vector, pBAV1K-T5, that produces varying quantities of active reporter proteins in Escherichia coli, Acinetobacter baylyi ADP1, Agrobacterium tumefaciens, (all gram-negative), Streptococcus pneumoniae, Leifsonia shinshuensis, Peanibacillus sp. S18-36 and Bacillus subtilis (gram-positive).
CONCLUSIONS/SIGNIFICANCE: Our results demonstrate the efficiency of pBAV1K-T5 replication in different bacterial species, thereby facilitating the study of proteins that don't fold well in E. coli and pathogens not amenable to existing genetic tools.
大多数质粒仅在特定的属或家族内复制。
方法/主要发现：在这里，我们描述了一种经过工程改造的高拷贝数表达载体 pBAV1K-T5，它在大肠杆菌、不动杆菌 ADP1、根瘤农杆菌（均为革兰氏阴性菌）、肺炎链球菌、信州利森氏菌、短芽孢杆菌 S18-36 和枯草芽孢杆菌（革兰氏阳性菌）中产生不同数量的活性报告蛋白。
结论/意义：我们的结果表明 pBAV1K-T5 在不同细菌物种中的复制效率，从而促进了对那些在大肠杆菌中不能很好折叠的蛋白质以及现有遗传工具不易处理的病原体的研究。
