Zhao Ge, Chen Min, Liu Ting, Sun Shi Y, Zhao Jing, Xie Li X
State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao - China.
Eur J Ophthalmol. 2012;22 Suppl 7:S40-5. doi: 10.5301/ejo.5000064.
To investigate whether the clinical characteristics of stromal herpetic simplex keratitis (HSK) are associated with herpes simplex virus type 1 (HSV-1) antigens distribution in the pathologic cornea.
Pathologic corneal buttons from 8 eyes were obtained during keratoplasty at the Shandong Eye Institute from 2006 to 2009. Immunohistochemical examination was performed to detect the distribution of HSV-1 antigens, including position, depth, and load in the cornea.
Each of the 8 pathologic corneal buttons was positive for HSV-1 antigen by immunohistochemical staining, and HSV-1 antigen was detected in the corneal stroma but not in the corneal epithelium or endothelium. Combined with the clinical characteristics, it was found that the distribution depth of HSV-1 antigen in the cornea was partly related to the disease course, and the load was related to the delay time for treatment. Furthermore, HSK could be effectively cured by deep anterior lamellar keratoplasty (DALK).
The distribution characteristics of HSV-1 in HSK corneal stroma supports the theory of HSV-1 latency in the cornea and guides the selection of DALK, rather than penetrating keratoplasty (PKP), for clinical stromal HSK treatment.
探讨单纯疱疹病毒性基质性角膜炎（HSK）的临床特征是否与病理角膜中单纯疱疹病毒1型（HSV-1）抗原的分布相关。
2006年至2009年在山东省眼科研究所进行角膜移植手术时，获取8只眼的病理角膜组织块。采用免疫组织化学检查检测HSV-1抗原在角膜中的分布情况，包括位置、深度及含量。
8个病理角膜组织块经免疫组织化学染色均显示HSV-1抗原阳性，HSV-1抗原在角膜基质中被检测到，而在角膜上皮或内皮中未被检测到。结合临床特征发现，HSV-1抗原在角膜中的分布深度与病程部分相关，含量与治疗延迟时间相关。此外，深板层角膜移植术（DALK）可有效治愈HSK。
HSV-1在HSK角膜基质中的分布特征支持HSV-1在角膜中潜伏的理论，并指导临床治疗基质性HSK时选择DALK而非穿透性角膜移植术（PKP）。
