需要初次穿透性角膜移植术的单纯疱疹性角膜炎的流行病学及分子分析
Epidemiology and molecular analysis of herpes simplex keratitis requiring primary penetrating keratoplasty.
作者信息
Branco B C, Gaudio P A, Margolis T P
机构信息
Department of Ophthalmology, Federal University of São Paulo, Brazil.
出版信息
Br J Ophthalmol. 2004 Oct;88(10):1285-8. doi: 10.1136/bjo.2003.040162.
AIMS
To determine whether herpes simplex keratitis (HSK) has declined as an indication for penetrating keratoplasty (PKP) at the University of California San Francisco (UCSF) over the past 30 years.
METHODS
Records of the Hogan Eye Pathology Laboratory were reviewed to determine the incidence of PKP performed for HSK from 1972 through 2001. Archived corneal tissue with the diagnosis of HSK was evaluated for herpes simplex virus (HSV) DNA by polymerase chain reaction (PCR) based assays.
RESULTS
The number of corneal buttons submitted with the clinical diagnosis of HSK decreased from 1972 to 2001, while the overall number of PKPs performed did not. The percentage of corneal buttons with a clinical diagnosis of HSK that contained detectable HSV DNA did not change over the course of the study period.
CONCLUSION
HSK declined as an indication for PKP from 1972 to 2001 at UCSF. It is unlikely that this decline was the result of improved diagnostic accuracy since detection of HSV DNA in corneal buttons with a clinical diagnosis of HSK was similar at the beginning and end of the study period.
目的
确定在过去30年里，单纯疱疹性角膜炎（HSK）作为加利福尼亚大学旧金山分校（UCSF）穿透性角膜移植术（PKP）的指征是否有所下降。
方法
回顾霍根眼病理实验室的记录，以确定1972年至2001年因HSK进行PKP的发生率。采用基于聚合酶链反应（PCR）的检测方法，对诊断为HSK的存档角膜组织进行单纯疱疹病毒（HSV）DNA评估。
结果
1972年至2001年，临床诊断为HSK的角膜植片数量减少，而PKP的总体数量未变。在研究期间，临床诊断为HSK且含有可检测到的HSV DNA的角膜植片百分比没有变化。
结论
1972年至2001年，在UCSF，HSK作为PKP的指征有所下降。由于在研究开始和结束时，临床诊断为HSK的角膜植片中HSV DNA的检测情况相似，因此这种下降不太可能是诊断准确性提高的结果。
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