Department of Rehabilitation, China-Japan Union Hospital, Jilin University, Changchun, China.
Orthop Surg. 2011 Nov;3(4):265-7. doi: 10.1111/j.1757-7861.2011.00155.x.
To compare the influence of different methods of adding vancomycin on the strength of bone cement used for fixing rabbits' femoral prostheses.
Eighteen New Zealand white rabbits (36 femora) were allocated to three groups of six rabbits (12 femora) each: a control group, an experimental group 1 and experimental group 2. The bone cement used in the control group was mixed manually without vancomycin; in experimental group 1 it was mixed manually with 5% (2 g/40 g) vancomycin; and in experimental group 2 it was mixed in a vacuum with 5% (2 g/40 g) vancomycin. The cement combinations were then used to fill the marrow cavity of rabbit femurs to fix prostheses. An electronic universal testing machine (EUTM) was used to test the pullout force of the prostheses with bone cement 48 hours later. Student's paired t-test was used for statistical analysis.
It was found that the maximum load of pullout force was 559.3 ± 82.3 N in the control group; 523.6 ± 86.5 N in experimental group 1; and 645.1 ± 105.9 N in experimental group 2. Statistical analysis showed there was no significant difference between the control group and experimental group 1 (P > 0.05). However, there were significant differences between the control group and experimental group 2 (P < 0.05); and between experimental group 1 and experimental group 2 (P < 0.05).
When bone cement and vancomycin were mixed manually in a low concentration (<5%) vancomycin had no influence on the strength of the bone cement. However, bone cement and vancomycin mixed at the same concentrations in a vacuum did have a statistically significant effect on the strength of the bone cement.
比较不同添加万古霉素方法对用于固定兔股骨假体的骨水泥强度的影响。
将 18 只新西兰大白兔（36 根股骨）分为三组，每组 6 只（12 根股骨）：对照组、实验组 1 和实验组 2。对照组的骨水泥混合时未添加万古霉素，实验组 1 手动混合时添加 5%（2 克/40 克）万古霉素，实验组 2 采用真空混合 5%（2 克/40 克）万古霉素。然后将水泥混合物填充到兔股骨的骨髓腔中以固定假体。48 小时后，使用电子万能试验机（EUTM）测试带骨水泥的假体的拔出力。采用学生配对 t 检验进行统计分析。
发现对照组的最大拔出力为 559.3±82.3N；实验组 1 为 523.6±86.5N；实验组 2 为 645.1±105.9N。统计分析显示，对照组与实验组 1 之间无显著差异（P＞0.05）。然而，对照组与实验组 2 之间有显著差异（P＜0.05）；实验组 1 与实验组 2 之间也有显著差异（P＜0.05）。
当骨水泥和万古霉素以低浓度（＜5%）混合时，手动混合对骨水泥强度没有影响。然而，在相同浓度下，骨水泥和万古霉素在真空中混合对骨水泥强度有统计学意义的影响。
