Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.
In Vivo. 2011 Nov-Dec;25(6):881-6.
Hepatocellular carcinoma (HCC) is one of the most lethal diseases, and one of the major causes of death in Japan. Our previous research of proteomics for cancerous and paired non-cancerous tissues from patients with HCC with hepatitis C virus infection (HCV-HCC) by means of the combination of two-dimensional gel electrophoresis (2-DE) and liquid chromatography tandem mass spectrometry (LC-MS/MS) reported that four of numerous spots of weaker intensity in cancerous tissues than in paired non-cancerous tissues were identified as four isoforms of liver type aldolase (aldolase B). In the present study, two-dimensional (2-D) Western blot analysis demonstrated a significantly lower expression of four isoforms of aldolase B in cancerous than in non-cancerous tissues.
Our finding of differences of expression aldolase B isoforms between cancerous and paired non-cancerous tissues for HCV-HCC may be useful for shedding light on some behaviors of aldolase B during hepatocellular carcinogenesis.
肝细胞癌（HCC）是最致命的疾病之一，也是日本主要的死亡原因之一。我们之前通过二维凝胶电泳（2-DE）和液相色谱串联质谱（LC-MS/MS）相结合的方法对丙型肝炎病毒感染（HCV-HCC）患者的肝癌和配对非癌组织进行蛋白质组学研究，报告说在癌组织中比配对非癌组织中强度较弱的许多斑点中有四个被鉴定为醛缩酶（aldolase B）的四个同工型。在本研究中，二维（2-D）Western blot 分析表明，癌组织中 aldolase B 的四个同工型的表达明显低于非癌组织。
我们发现 HCV-HCC 癌组织和配对非癌组织中 aldolase B 同工型表达的差异，可能有助于阐明 aldolase B 在肝细胞癌变过程中的某些行为。
