叶酸补充对大鼠氧化胃黏膜损伤和胃酸分泌反应的影响。
Effect of folic acid supplementation on oxidative gastric mucosa damage and acid secretory response in the rat.
机构信息
Department of Physiology, School of Basic Medical Sciences, Igbinedion University, Okada, Nigeria.
出版信息
Indian J Pharmacol. 2011 Sep;43(5):578-81. doi: 10.4103/0253-7613.84976.
OBJECTIVE
This study investigated the antioxidative and antisecretory properties of folic acid in the rats' stomach.
MATERIALS AND METHODS
Male Wistar rats were treated with 2 mg/kg diet of folic acid for 21 days. Gastric ulceration was induced by indomethacin, scored, and assayed to determine the concentration of total protein, mucus, malondialdehyde (MDA), catalase (CAT) and superoxide dismutase (SOD) in homogenized samples. Normal saline and Ranitidine treated group served as negative and positive control, respectively. Basal and stimulated acid secretion was measured by continuous perfusion method.
RESULT
Indomethacin caused severe damage to the rats' stomach with an ulcer index of 4.32 ± 0.13, increase in MDA concentration and reduction in the concentration of protein, mucus, catalase and superoxide dismutase (P < 0.001). Pre-treatment with folic acid prevented the formation of ulcers by 32%, and attenuated the inhibition of mucus by 14%, CAT, 51% and SOD, 150%. Ranitidine afforded 56% prevention in ulcer formation with 67%, 55% and 78% attenuation of the inhibition of mucus, CAT and SOD, respectively, by indomethacin. While indomethacin-induced lipid peroxidation was attenuated by 58% reduction in MDA concentration on pretreatment with folic acid, Ranitidine offered 65% reduction. Basal and stimulated acid secretions were significantly reduced in the treated when compared with control animals. Folic acid produced a 21% reduction in the basal acid output when compared with the control animals (P < 0.05), and 140% reduction in histamine-induced acid response.
CONCLUSION
The results indicate the gastroprotective activity of folic acid due its antioxidative and anti-secretory properties.
目的
本研究旨在探讨叶酸在大鼠胃中的抗氧化和抗分泌作用。
材料和方法
雄性 Wistar 大鼠给予 2mg/kg 饮食叶酸 21 天。用吲哚美辛诱导胃溃疡，评分并测定匀浆样品中总蛋白、黏液、丙二醛（MDA）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的浓度。生理盐水和雷尼替丁处理组分别作为阴性和阳性对照。采用连续灌注法测定基础和刺激胃酸分泌。
结果
吲哚美辛对大鼠胃造成严重损伤，溃疡指数为 4.32±0.13，MDA 浓度升高，蛋白质、黏液、CAT 和 SOD 浓度降低（P＜0.001）。叶酸预处理可使溃疡形成减少 32%，黏液抑制减少 14%，CAT 减少 51%，SOD 减少 150%。雷尼替丁可使溃疡形成减少 56%，对吲哚美辛抑制黏液、CAT 和 SOD 的作用分别减弱 67%、55%和 78%。叶酸预处理可使吲哚美辛诱导的脂质过氧化作用减弱 58%，MDA 浓度降低 65%。与对照组相比，吲哚美辛处理组基础和刺激胃酸分泌均显著减少。与对照组相比，叶酸使基础胃酸分泌减少 21%（P＜0.05），组胺诱导的胃酸反应减少 140%。
结论
结果表明，叶酸具有抗氧化和抗分泌作用，具有胃保护活性。
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