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在无血清培养基中培养加拿大支原体和羞怯支原体的条件。

Conditions for growing Mycoplasma canadense and Mycoplasma verecundum in a serum-free medium.

作者信息

Muñoz G, Sotomayor P

机构信息

Laboratorio de Genética Molecular Instituto de Biología, Facultad de Ciencias Básicas y Matemáticas, Universidad Católica de Valparaíso, Chile.

出版信息

Appl Environ Microbiol. 1990 Jul;56(7):2259-61. doi: 10.1128/aem.56.7.2259-2261.1990.

DOI:10.1128/aem.56.7.2259-2261.1990
PMID:2202260
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC184595/
Abstract

Mycoplasma canadense and Mycoplasma verecundum were cultured in a serum-free medium containing bovine serum albumin, cholesterol, oleic acid, and palmitic acid in order to avoid the addition of horse serum. Growth was detected by measurement of A640 and by colony formation. The level of growth attained in this medium was less than that obtained in the horse serum-supplemented media, but colonies retained their distinctive morphology.

摘要

加拿大支原体和羞怯支原体在含有牛血清白蛋白、胆固醇、油酸和棕榈酸的无血清培养基中培养，以避免添加马血清。通过测量A640和菌落形成来检测生长情况。在这种培养基中达到的生长水平低于在添加马血清的培养基中获得的水平，但菌落保留了其独特的形态。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1009/184595/eba5e5a96a83/aem00088-0289-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1009/184595/932d13532838/aem00088-0288-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1009/184595/eba5e5a96a83/aem00088-0289-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1009/184595/932d13532838/aem00088-0288-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1009/184595/eba5e5a96a83/aem00088-0289-a.jpg

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本文引用的文献

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Morphogenesis of Mycoplasma and bacterial L-form colonies.支原体和细菌L型菌落的形态发生
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Control of membrane lipids in Mycoplasma gallisepticum: effect on lipid order.鸡毒支原体中膜脂的调控：对脂序的影响。
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Distribution and movement of sterols with different side chain structures between the two leaflets of the membrane bilayer of mycoplasma cells.具有不同侧链结构的固醇类物质在支原体细胞膜双层的两个小叶之间的分布和移动。
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Kinetics of cholesterol and phospholipid exchange between Mycoplasma gallisepticum cells and lipid vesicles. Alterations in membrane cholesterol and protein content.鸡毒支原体细胞与脂质囊泡之间胆固醇和磷脂交换的动力学。膜胆固醇和蛋白质含量的变化。
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Liposomes replace serum for cultivation of fermenting mycoplasmas.脂质体替代血清用于培养发酵型支原体。
Appl Environ Microbiol. 1983 Aug;46(2):370-4. doi: 10.1128/aem.46.2.370-374.1983.
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The supply of cholesterol and fatty acids for the growth of mycoplasmas.用于支原体生长的胆固醇和脂肪酸供应。
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Cholesterol requirement of mycoplasmas.支原体的胆固醇需求
J Bacteriol. 1970 May;102(2):306-10. doi: 10.1128/jb.102.2.306-310.1970.
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Mycoplasma verecundum, a new species isolated from bovine eyes.温和支原体，一种从牛眼分离出的新物种。
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10
Comparison of the efficacy of egg yolk extract and horse serum for growth of Mycoplasma pneumoniae.蛋黄提取物和马血清对肺炎支原体生长的功效比较。
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