Le Grimellec C, Cardinal J, Giocondi M C, Carrière S
J Bacteriol. 1981 Apr;146(1):155-62. doi: 10.1128/jb.146.1.155-162.1981.
Adaptation of Mycoplasma gallisepticum, a sterol-requiring Mycoplasma sp., to growth in a serum-free medium supplemented with cholesterol in decreasing concentrations and with various saturated or unsaturated fatty acids enabled us to control both the cholesterol levels and the membrane fatty acid composition. An estimate of the membrane physical state from fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene indicated that the membrane lipids of native M. gallisepticum were highly ordered. Elongation of the saturated fatty acid chains from 14 to 18 carbon atoms caused only a small increase in the membrane lipid ordering, whereas the introduction of a cis double bond reduced it significantly. Lipid-phase transitions were observed in low-cholesterol-adapted organisms, whose membrane lipids were still highly ordered at the growth temperature.
鸡毒支原体是一种需要固醇的支原体，使其适应在添加了浓度逐渐降低的胆固醇以及各种饱和或不饱和脂肪酸的无血清培养基中生长，这使我们能够控制胆固醇水平和膜脂肪酸组成。通过1,6-二苯基-1,3,5-己三烯的荧光偏振对膜物理状态进行估计，结果表明天然鸡毒支原体的膜脂质高度有序。饱和脂肪酸链从14个碳原子延长至18个碳原子仅使膜脂质有序性略有增加，而引入顺式双键则使其显著降低。在低胆固醇适应型生物体中观察到脂质相转变，其膜脂质在生长温度下仍高度有序。
