Small Animal Medicine Clinic, LMU Munich, München, Germany.
Lett Appl Microbiol. 2012 Jan;54(1):26-31. doi: 10.1111/j.1472-765X.2011.03167.x. Epub 2011 Nov 18.
Commercially available selective media for methicillin-resistant Staphylococcus aureus (MRSA) were tested for the detection and isolation of methicillin-resistant Staphylococcus pseudintermedius (MRSP).
Five different screening agars [mannitol salt agar with oxacillin and BD BBL™ Chromagar™ MRSA (BD Diagnostics); chromID™ MRSA agar (bioMérieux); Oxacillin resistance screening agar base (ORSAB); and Brilliance MRSA agar (Oxoid)] were analysed for the detection of MRSP. Bacteria that may be isolated together with MRSP and may grow on the screening agars were included in the study to determine possible interference with the growth of MRSP. MRSP grew well on all selective media except on BD BBL™ Chromagar™ MRSA (BD Diagnostics) and chromID™ MRSA agar (bioMérieux), on which a low to moderate growth rate was noted.
ORSAB (Oxoid) and Brilliance MRSA agar (Oxoid) are most suitable for the detection and isolation of MRSP from clinical material.
The importance of MRSP in veterinary medicine is increasing. Diagnostic systems are needed to detect MRSP carrier as soon as possible. This study provides information about selected MRSA screening agars for the detection of MRSP to the clinical microbiologists.
测试市售耐甲氧西林金黄色葡萄球菌（MRSA）选择性培养基，以检测和分离耐甲氧西林中间葡萄球菌（MRSP）。
分析了五种不同的筛选琼脂[含苯唑西林和 BD BBL™ Chromagar™ MRSA（BD 诊断）的甘露醇盐琼脂；chromID™ MRSA 琼脂（生物梅里埃）；Oxacillin resistance screening agar base（ORSAB）；和 Brilliance MRSA 琼脂（Oxoid）]，以检测 MRSP。本研究纳入了可能与 MRSP 一起分离并在筛选琼脂上生长的细菌，以确定其对 MRSP 生长的可能干扰。MRSP 在所有选择性培养基上生长良好，除了在 BD BBL™ Chromagar™ MRSA（BD 诊断）和 chromID™ MRSA 琼脂（生物梅里埃）上，其生长速度较低或中等。
ORSAB（Oxoid）和 Brilliance MRSA 琼脂（Oxoid）最适合从临床标本中检测和分离 MRSP。
MRSP 在兽医医学中的重要性正在增加。需要诊断系统尽快检测 MRSP 携带者。本研究为临床微生物学家提供了有关 MRSA 筛选琼脂检测 MRSP 的信息。
