Xu Zhenbo, Hou Yuchao, Peters Brian M, Chen Dingqiang, Li Bing, Li Lin, Shirtliff Mark E
College of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, China.
Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, MD, 21201, USA.
Mol Biol Rep. 2016 Nov;43(11):1205-1212. doi: 10.1007/s11033-016-4062-3. Epub 2016 Aug 25.
For the past decade, a number of chromogenic media for methicillin-resistance Staphylococcus aureus (MRSA) detection have been developed and applied, including Oxoid Brilliance™ MRSA, CHROMagar™ MRSA, BBL™ CHROMagar™ MRSA, MRSASelect and chromID MRSA. The advantages of these chromogenic media offers direct detection of visible staphylococcal colonies, coupled with the use of chromogenic enzymatic substrates that can be hydrolyzed by S. aureus to confirm species or strain identification. BBL™ CHROMagar™ MRSA and MRSASelect are designed for detection of nasal colonization by MRSA, while CHROMagar™ MRSA, Oxoid Brilliance™ MRSA and chromID MRSA are readily applied in bacterial screening. This review summarizes the characteristics, principles and capacities of these selective media, and focuses on comparison of different chromogenic media.
在过去十年中，已经开发并应用了多种用于检测耐甲氧西林金黄色葡萄球菌（MRSA）的显色培养基，包括Oxoid Brilliance™ MRSA、CHROMagar™ MRSA、BBL™ CHROMagar™ MRSA、MRSASelect和chromID MRSA。这些显色培养基的优点是能够直接检测可见的葡萄球菌菌落，同时使用可被金黄色葡萄球菌水解的显色酶底物来确认菌种或菌株鉴定。BBL™ CHROMagar™ MRSA和MRSASelect设计用于检测MRSA的鼻腔定植情况，而CHROMagar™ MRSA、Oxoid Brilliance™ MRSA和chromID MRSA则易于应用于细菌筛查。本综述总结了这些选择性培养基的特点、原理和性能，并着重比较了不同的显色培养基。
