Kuratani S, Bockman D E
Department of Anatomy, Medical College of Georgia, Augusta.
Arch Histol Cytol. 1990 Jul;53(3):267-73. doi: 10.1679/aohc.53.267.
The purpose of this study was to correlate the contributions by derivatives of the neural crest with the development of the epithelial primordium of the thymus. The monoclonal antibody E/C8 was used to localize derivatives of the neural crest in chick embryos. Neural crest was ablated by microcautery of neural folds. Evaluation of thymic development was carried out on serial sections of embryos sacrificed on the sixth day of incubation. The size of the epithelial thymic primordium was smaller in experimental animals than in shams. E/C8-immunoreactivity was concentrated around the periphery of the primordium. It was determined, by quantifying reaction product using the Core-SCAN computer color analysis program, that the amount of immunoreactivity was decreased after ablation of neural crest. Statistical analysis showed that the quantity of reaction product was positively and significantly correlated with the size of the thymic primordium. It is concluded that mesenchymal derivatives of the neural crest, through participation in the early development of the epithelial primordium, play an important role in thymic development, and therefore with development of the immune system.
本研究的目的是将神经嵴衍生物的作用与胸腺上皮原基的发育联系起来。使用单克隆抗体E/C8对鸡胚中的神经嵴衍生物进行定位。通过对神经褶进行微烧灼来去除神经嵴。对孵化第6天处死的胚胎的连续切片进行胸腺发育评估。实验动物的上皮胸腺原基比假手术动物的小。E/C8免疫反应性集中在原基周边。通过使用Core-SCAN计算机颜色分析程序对反应产物进行定量测定，发现去除神经嵴后免疫反应性的量减少。统计分析表明，反应产物的量与胸腺原基的大小呈正相关且具有显著性。得出的结论是，神经嵴的间充质衍生物通过参与上皮原基的早期发育，在胸腺发育中发挥重要作用，因此也与免疫系统的发育有关。
