State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, People's Republic of China.
Acta Biomater. 2012 Feb;8(2):904-15. doi: 10.1016/j.actbio.2011.09.031. Epub 2011 Sep 29.
In this work, zinc was incorporated into TiO2 coatings on titanium by plasma electrolytic oxidation to obtain the implant with good bacterial inhibition ability and bone-formability. The porous and nanostructured Zn-incorporated TiO2 coatings are built up from pores smaller than 5 μm and grains 20-100 nm in size, in which the element Zn exists as ZnO. The results obtained from the antibacterial studies suggest that the Zn-incorporated TiO2 coatings can greatly inhibit the growth of both Staphylococcus aureus and Escherichia coli, and the ability to inhibit bacteria can be improved by increasing the Zn content in the coatings. Moreover, the in vitro cytocompatibility evaluation demonstrates that the adhesion, proliferation and differentiation of rat bone marrow stem cells (bMSC) on Zn-incorporated coatings are significantly enhanced compared with Zn-free coating and commercially pure Ti plate, and no cytotoxicity appeared on any of the Zn-incorporated TiO2 coatings. Moreover, bMSC express higher level of alkaline phosphatase activity on Zn-incorporated TiO2 coatings and are induced to differentiate into osteoblast cells. The better antibacterial activity, cytocompatibility and the capability to promote bMSC osteogenic differentiation of Zn-incorporated TiO2 coatings may be attributed to the fact that Zn ions can be slowly and constantly released from the coatings. In conclusion, innovative Zn-incorporated TiO2 coatings on titanium with excellent antibacterial activity and biocompatibility are promising candidates for orthopedic and dental implants.
在这项工作中，通过等离子体电解氧化在钛上掺入锌，以获得具有良好抑菌能力和骨形成能力的植入物。多孔和纳米结构的锌掺杂 TiO2 涂层由小于 5μm 的孔和 20-100nm 大小的晶粒组成，其中元素锌以 ZnO 的形式存在。抑菌研究结果表明，锌掺杂 TiO2 涂层能显著抑制金黄色葡萄球菌和大肠杆菌的生长，且涂层中锌含量的增加可以提高抑菌能力。此外，体外细胞相容性评价表明，与无锌涂层和商用纯钛板相比，锌掺杂涂层上大鼠骨髓基质细胞（bMSC）的黏附、增殖和分化明显增强，且任何锌掺杂 TiO2 涂层均无细胞毒性。此外，bMSC 在锌掺杂 TiO2 涂层上表达更高水平的碱性磷酸酶活性，并被诱导分化为成骨细胞。锌掺杂 TiO2 涂层具有更好的抑菌活性、细胞相容性和促进 bMSC 成骨分化的能力，可能归因于锌离子可以从涂层中缓慢且持续地释放。总之，具有优异抗菌活性和生物相容性的新型钛基锌掺杂 TiO2 涂层有望成为骨科和牙科植入物的候选材料。
