Department of Pharmaceutical Sciences, Meiji Pharmaceutical University, Tokyo, Japan.
Colloids Surf B Biointerfaces. 2012 Feb 1;90:58-61. doi: 10.1016/j.colsurfb.2011.09.046. Epub 2011 Oct 5.
The presence/absence of adsorption of 9 representative types of antimicrobial agent used in combination with a polymyxin-B immobilized fiber (PMX-F) were determined and the degree of adsorption to PMX-F was quantitatively evaluated. Various antimicrobial agents were dissolved at appropriate concentrations, and PMX-F was added to each solution and incubated at 37°C. Antimicrobial solutions without PMX-F were also similarly incubated as controls. After 2 and 4h, the concentration of each antimicrobial agent was determined using HPLC. To produce an environment closer to the in vivo state, albumin or serum was added, and similar evaluation was performed. In the presence of albumin, the rate of adsorption to PMX-F was relatively high for Cefmetazon(®), Pentcillin(®), Ciproxan(®) and Zyvox(®). In the presence of serum, the adsorption rate was 4.02±2.83% for Pentcillin(®), 5.59±1.00% for Ciproxan(®), and 22.12±3.23% for Zyvox(®). The results of this study suggest that adequate caution is necessary on the clinical use of Zyvox(®), which was adsorbed in the presence of serum as an environment close to the in vivo environment, but the use of other antimicrobial agents in combination with PMX-F may have only slight influences on adsorption to PMX-F.
本研究采用高效液相色谱法（HPLC）测定了 9 种常用的与多粘菌素 B 固定化纤维（PMX-F）联合使用的抗菌药物在 PMX-F 上的吸附情况，并定量评估了其吸附程度。将各种抗菌药物溶解在适当的浓度下，向每个溶液中加入 PMX-F，并在 37°C 下孵育。同样孵育不含 PMX-F 的抗菌溶液作为对照。孵育 2 和 4 小时后，使用 HPLC 测定每种抗菌药物的浓度。为了更接近体内环境，加入白蛋白或血清，并进行类似的评估。在白蛋白存在的情况下，头孢美唑(®)、青霉素(®)、西普罗先(®)和利奈唑胺(®)在 PMX-F 上的吸附率相对较高。在血清存在的情况下，青霉素(®)的吸附率为 4.02±2.83%，西普罗先(®)为 5.59±1.00%，利奈唑胺(®)为 22.12±3.23%。本研究结果表明，在接近体内环境的血清存在的情况下，利奈唑胺(®)被吸附，因此在临床使用时需要谨慎。然而，其他抗菌药物与 PMX-F 联合使用可能对 PMX-F 的吸附影响较小。
