Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Daejeon 34054, Korea.
Korean Convergence Medicine Major KIOM, University of Science & Technology (UST), Daejeon 34054, Korea.
Molecules. 2021 Dec 28;27(1):169. doi: 10.3390/molecules27010169.
bulbus has been widely used to treat symptoms of coughs and airway congestion in the chest due to pathological colds and damp phlegm in traditional Chinese medicine. Despite its long history of traditional use, its pharmacological activities on osteoclastogenesis and osteoporosis have not been evaluated. This study investigated the effects of the water extract of bulbus (WEFT) on osteoclast differentiation in bone marrow-derived macrophage cells and on ovariectomy (OVX)-induced osteoporosis in mice. We found that WEFT significantly inhibited osteoclastogenesis by downregulating the receptor activator of the NF-κB ligand (RANKL) signaling-induced nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) expression. In an OVX-induced osteoporosis model, WEFT significantly prevented the OVX-induced trabecular loss of femurs, accompanied by a reduction in fat accumulation in the bone marrow and liver. In addition, WEFT significantly prevented weight gain and gonadal fat gain without recovering uterine atrophy. Using ultrahigh-performance liquid chromatography-tandem mass spectrometry, seven alkaloids (peimisine glucoside, yibeissine, peiminoside, sipeimine-glucoside, peimisine, peimine, and peiminine) were identified in WEFT. The results of this study suggest that WEFT can be a potential pharmacological candidate to reduce menopausal osteoporosis and menopause-related symptoms, such as fat accumulation.
百合在中医药中被广泛用于治疗因病理感冒和湿痰引起的咳嗽和气道充血等症状。尽管其在传统用途上历史悠久，但尚未评估其对破骨细胞生成和骨质疏松症的药理活性。本研究调查了百合水提取物（WEFT）对骨髓来源的巨噬细胞中破骨细胞分化以及去卵巢（OVX）诱导的小鼠骨质疏松症的影响。我们发现 WEFT 通过下调核因子活化 T 细胞胞浆 1（NFATc1）表达来显著抑制破骨细胞生成。在 OVX 诱导的骨质疏松症模型中，WEFT 可显著防止 OVX 引起的股骨小梁丢失，同时减少骨髓和肝脏中的脂肪积累。此外，WEFT 可显著防止体重增加和性腺脂肪增加，而不会恢复子宫萎缩。使用超高效液相色谱-串联质谱法，在 WEFT 中鉴定出七种生物碱（百合苷葡萄糖苷、伊贝辛、百合苷、斯佩米苷葡萄糖苷、百合碱、佩米嗪和佩米宁）。本研究结果表明，WEFT 可能是一种潜在的药理学候选药物，可减少绝经后骨质疏松症和与绝经相关的症状，如脂肪积累。
