Department Respiratory, Qilu Hospital of Shandong University, Jinan, China.
Yonsei Med J. 2011 Nov;52(6):953-60. doi: 10.3349/ymj.2011.52.6.953.
Polymorphisms of several candidate genes have been studied and associated with the development of chronic obstructive pulmonary disease (COPD). One such candidate is the SERPINE2 (Serpin peptidase inhibitor, clade E member 2) gene.
To assess whether the SERPINE2 gene is associated with COPD in a Chinese Han population. Samples were collected from a Chinese Han population and analyzed for the association of single nucleotide polymor phisms (SNPs) or haplotypes of SERPINE2 gene with COPD in a case-control study. Three SNPs including rs840088 G/A in intron 1, rs1438831 A/G in 5' upstream sequence and rs3795879 G/A in intron 3 were detected using the polymerase chain reaction (PCR)-based restriction fragment length polymorphism technique in 409 COPD subjects and 411 controls. Genotyping of the SREPINE2 polymorphisms at positions rs840088, rs1438831and rs3795879 was performed.
We found that none of the rs840088G/A, rs1438831G/A and rs3795879 G/A polymorphisms were associated with the disease. The p-values were 0.630, 0.208 and 0.398 respectively.
Our data suggested that there was no significant association between SERPINE2 polymorphism and COPD susceptibility in the Chinese Han population.
许多候选基因的多态性已被研究并与慢性阻塞性肺疾病（COPD）的发展相关。其中一个候选基因是丝氨酸蛋白酶抑制剂，凝血酶原激活物抑制剂成员 2（SERPINE2）基因。
评估 SERPINE2 基因是否与中国汉族人群中的 COPD 相关。采集了来自中国汉族人群的样本，并在病例对照研究中分析了 SERPINE2 基因的单核苷酸多态性（SNP）或单倍型与 COPD 的关联。使用聚合酶链反应（PCR）-基于限制片段长度多态性技术检测了包括内含子 1 中的 rs840088G/A、5'上游序列中的 rs1438831A/G 和内含子 3 中的 rs3795879G/A 在内的三个 SNP。对 COPD 患者 409 例和对照组 411 例的 SREPINE2 多态性 rs840088、rs1438831 和 rs3795879 进行了基因分型。
我们发现，rs840088G/A、rs1438831G/A 和 rs3795879G/A 多态性均与疾病无关。其 p 值分别为 0.630、0.208 和 0.398。
我们的数据表明，在中国汉族人群中，SERPINE2 多态性与 COPD 易感性之间没有显著关联。
