Department of Infectious Diseases and Research Laboratory of Infectious Diseases, Children's Hospital of Fudan University, Shanghai, China.
J Med Virol. 2012 Jan;84(1):145-51. doi: 10.1002/jmv.22248. Epub 2011 Oct 25.
A prospective investigation was carried out among pediatric outpatients and inpatients with acute non-dysenteric diarrhea between August, 2008 and July, 2009 in Shanghai, Hangzhou, Chongqing, and Tianjin, China. One step real-time RT-PCR was used for detection of norovirus (NoV) genogroups I and II (GI, GII). The NoV genotypes were classified based on partial capsid sequences. Rotavirus (RV) was detected in parallel. Among 4,123 fecal samples from outpatients, 1,067 (25.9%) were NoV-positive, of which 1,051 (98.5%) belonged to GII and 1,309 (31.7%) were RV-positive. In the inpatient group (n = 317), 25.6% were NoV-positive and 41.6% were RV-positive. Four hundred and fifty-one out of 1,067 NoV-positive strains were sequenced and genotyped and 6 typed strains were GI (3 GI.3, 2 GI.5, 1 GI.4) and 445 typed strains were GII. GII strains clustered into nine genotypes including GII.4 2006b (69.2%), the only GII.4 variant identified in this study, followed by GII.3 (23.8%), GII.6 (3.6%), GII.12 (1.3%), GII.2 (0.9%), GII.13 (0.4%), GII.14 (0.2%), GII.7 (0.2%), and GII.16 (0.2%). A peak of NoV infections was observed during the cold season in Tianjin, while NoV activity was higher between late summer and autumn and lower during winter in Shanghai, Hangzhou, and Chongqing. NoV is a common causative agent of childhood diarrhea in China and the seasons of NoV-associated diarrhea varies between regions. The results show that NoV GII.4 2006b was the predominant strain circulating in China between 2008 and 2009.
2008 年 8 月至 2009 年 7 月期间，在中国上海、杭州、重庆和天津，对儿科门诊和住院急性非痢疾性腹泻患者进行了前瞻性研究。采用一步实时 RT-PCR 检测诺如病毒（NoV）基因 I 和 II 型（GI、GII）。根据部分衣壳序列对 NoV 基因型进行分类。同时检测轮状病毒（RV）。在来自门诊患者的 4123 份粪便样本中，有 1067 份（25.9%）为 NoV 阳性，其中 1051 份（98.5%）属于 GII，1309 份（31.7%）为 RV 阳性。在住院患者组（n=317）中，25.6%为 NoV 阳性，41.6%为 RV 阳性。对 1067 份 NoV 阳性株中的 451 株进行了测序和基因分型，其中 6 株为 GI（3 株 GI.3、2 株 GI.5、1 株 GI.4），445 株为 GII。GII 株分为 9 种基因型，包括本研究中唯一鉴定到的 GII.4 2006b，其次是 GII.3（23.8%）、GII.6（3.6%）、GII.12（1.3%）、GII.2（0.9%）、GII.13（0.4%）、GII.14（0.2%）、GII.7（0.2%）和 GII.16（0.2%）。在天津，诺如病毒感染高峰出现在寒冷季节，而在上海、杭州和重庆，诺如病毒活动在夏末至秋季较高，冬季较低。诺如病毒是中国儿童腹泻的常见病原体，诺如病毒相关性腹泻的季节在不同地区有所不同。结果表明，NoV GII.4 2006b 是 2008 年至 2009 年期间中国流行的主要毒株。
