Division of Laboratory Science, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, NE, Mailstop F-47, Atlanta, GA 30341, USA.
Cancer Epidemiol Biomarkers Prev. 2012 Jan;21(1):39-44. doi: 10.1158/1055-9965.EPI-11-0800. Epub 2011 Oct 25.
Benzo[a]pyrene (BaP) is one of the most potent carcinogens generated in cigarette smoke. During smoking, cigarette filters trap a significant portion of mainstream smoke benzo[a]pyrene. This trapped portion is proportional to what exits the end of the filter and is drawn into the mouth of smokers.
We developed a new method to estimate mouth-level BaP intake using filter analysis. In this analysis, cigarettes are smoked by a smoking machine using a variety of conditions to yield a range of mainstream smoke deliveries, which approximate a range of human puffing characteristics. Mainstream smoke BaP collected on Cambridge filter pads and the corresponding 1-cm mouth-end cigarette filter butts is extracted, purified by solid-phase extraction, and quantified by high-performance liquid chromatography coupled with a fluorescence detector. On the basis of the amount of BaP retained in cigarette butts and the amount collected on pads, we can relate them using a linear regression model.
Using this model and subsequently analyzing cigarette filters collected from smokers, we are able to estimate their mouth-level intakes, which smokers received when they consumed cigarettes. We made a series of measurements using research cigarettes and select commercial cigarettes having a wide range of machine smoke "tar" and nicotine deliveries.
In all cases, results indicate a linear relation of BaP between cigarette filter butts and Cambridge filter pads, with R(2) ranging from 0.93 to 0.98.
This technique provides a noninvasive means to examine intake on a per cigarette basis to examine both exposure and behavioral aspects of smoking.
苯并[a]芘（BaP）是香烟烟雾中产生的最具致癌性的物质之一。在吸烟过程中，香烟过滤嘴会截留主流烟雾中的很大一部分苯并[a]芘。截留的部分与从过滤嘴末端逸出并被吸烟者吸入的部分成正比。
我们开发了一种使用过滤嘴分析来估计口吸苯并[a]芘摄入量的新方法。在这种分析中，香烟通过吸烟机以各种条件吸烟，产生一系列主流烟雾传递，模拟人类吸烟的各种特点。在剑桥过滤垫上收集的主流烟雾 BaP 和相应的 1 厘米烟嘴过滤嘴末端的香烟过滤嘴被提取、固相萃取纯化，并通过高效液相色谱与荧光检测器定量。基于过滤嘴中保留的 BaP 量和垫上收集的 BaP 量，我们可以使用线性回归模型将它们联系起来。
使用该模型，然后分析从吸烟者那里收集的香烟过滤嘴，我们能够估计他们在吸食香烟时所接受的口吸摄入量。我们使用研究香烟和具有广泛机器烟雾“焦油”和尼古丁传递范围的选定商业香烟进行了一系列测量。
在所有情况下，结果表明香烟过滤嘴和剑桥过滤垫之间的 BaP 呈线性关系，R² 范围从 0.93 到 0.98。
这种技术提供了一种非侵入性的方法，可以根据每支香烟的摄入量来检查暴露和吸烟行为方面的情况。
