Nagashima Yuji, Matsumoto Takuya, Kadoyama Keisuke, Ishizaki Shoichiro, Terayama Makoto
Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.
J Toxicol. 2011;2011:801285. doi: 10.1155/2011/801285. Epub 2011 Oct 19.
Green toadfish Lagocephalus lunaris inhabits tropical and subtropical seas and contains high tetrodotoxin (TTX) levels in the muscle as well as liver and gonad. In 2008 to 2009, food poisoning due to ingesting L. lunais occurred in Western Japan. Five specimens of green toadfish caught in Kyushu coast, Japan, were analyzed for toxicity, toxins, and species identification. All five specimens were toxic by bioassay. Comparing the maximum toxicity in tissues, ovary contained the most toxin (1810 mouse unit [MU]/g), followed by liver (341 MU/g), muscle (135 MU/g), skin (79 MU/g), and intestine (72 MU/g). Liquid chromatography/mass spectrometry analysis revealed that TTX was the major toxin. Nucleotide sequence analysis of the 16S rRNA gene fragment of muscle mitochondrial DNA indicated that partial sequences of PCR products of four specimens were identical with that of L. lunaris. The sequence of one specimen was indistinguishable from that of the brown-backed toadfish Lagocephalus wheeleri, a nontoxic species.
绿鳍马面鲀栖息于热带和亚热带海域，其肌肉、肝脏和性腺中含有高浓度的河豚毒素（TTX）。2008年至2009年，日本西部发生了因食用绿鳍马面鲀导致的食物中毒事件。对在日本九州海岸捕获的5条绿鳍马面鲀标本进行了毒性、毒素及物种鉴定分析。通过生物测定，所有5条标本均有毒性。比较各组织中的最大毒性，卵巢含毒素最多（1810小鼠单位[MU]/克），其次是肝脏（341 MU/克）、肌肉（135 MU/克）、皮肤（79 MU/克）和肠道（72 MU/克）。液相色谱/质谱分析表明，TTX是主要毒素。对肌肉线粒体DNA的16S rRNA基因片段进行核苷酸序列分析，结果显示4条标本的PCR产物部分序列与绿鳍马面鲀的相同。1条标本的序列与无毒物种褐背马面鲀无法区分。
