Shiomi K, Inaoka H, Yamanaka H, Kikuchi T
Toxicon. 1985;23(2):331-6. doi: 10.1016/0041-0101(85)90156-4.
The toxins in the muscle, skin and liver of the puffer fish Lagocephalus lunaris lunaris and those in the skin and liver of another puffer fish Fugu niphobles were purified by successive column chromatography on activated charcoal, Bio-Gel P-2 and Bio-Rex 70 and analyzed by electrophoresis and thin layer chromatography. Although, regardless of the tissues and species, tetrodotoxin was predominant and accounted for more than 90% of the total toxins, the following minor toxins were newly detected; toxin A and toxin B in L. lunaris lunaris and toxin C in F. niphobles. Since these minor toxins, like tetrodotoxin, were positive to the Weber reagent and 10% KOH, but negative to 1% H2O2, they were assumed to be tetrodotoxin-like compounds. Interestingly, the electrophoretic and thin layer chromatographic analyses showed that toxin A and toxin C are probably identical with AFT3 and AFT1, the minor toxins in the crab Atergatis floridus from Chiba, Japan, respectively.
对月腹刺鲀的肌肉、皮肤和肝脏以及另一河豚黄鳍东方鲀的皮肤和肝脏中的毒素，通过在活性炭、Bio-Gel P-2和Bio-Rex 70上连续进行柱色谱法进行纯化，并通过电泳和薄层色谱法进行分析。尽管无论组织和物种如何，河豚毒素均占主导地位，占总毒素的90%以上，但新检测到以下次要毒素：月腹刺鲀中的毒素A和毒素B以及黄鳍东方鲀中的毒素C。由于这些次要毒素与河豚毒素一样，对韦伯试剂和10%氢氧化钾呈阳性反应，但对1%过氧化氢呈阴性反应，因此假定它们为类河豚毒素化合物。有趣的是，电泳和薄层色谱分析表明，毒素A和毒素C可能分别与来自日本千叶的花纹爱洁蟹中的次要毒素AFT3和AFT1相同。
