Shahed A R, Miller A, Chalker D, Allmann D W
J Cyclic Nucleotide Res. 1979;5(1):43-53.
The effect of NaF on cAMP production was studied in hepatocytes isolated from fed and fasted rats. A four-six fold increase in hepatocyte cAMP production was observed in the presence of 10-20 mM NaF in cells isolated from either fed or fasted rats. The maximal stimulation of cAMP production was observed after a 10 min incubation in the presence of 1 mM theophylline. However, as little as 0.05-0.15 mM NaF induced a significant increase in cAMP production. It was also found that NaF would alter the production of glucose in isolated rat hepatocytes. When hepatocytes from fed rats were incubated with 0.05-5 mM NaF there was an increase in amount of glucose released from endogenous sources. Also NaF resulted in a decrease in lactate and pyruvate production. Similarly NaF stimulated glucose production in hepatocytes from fasted rats. The maximal stimulation was observed with about 0.15-0.25 mM NaF. At NaF concentrations greater than 1.5 mM a decrease in glucose production was observed. It is concluded that NaF increases the level of cAMP and alters glucose metabolism in intact hepatocytes.
研究了氟化钠（NaF）对从喂食和禁食大鼠分离的肝细胞中cAMP产生的影响。在从喂食或禁食大鼠分离的细胞中，当存在10 - 20 mM NaF时，观察到肝细胞cAMP产生增加了4至6倍。在1 mM茶碱存在下孵育10分钟后，观察到cAMP产生的最大刺激。然而，低至0.05 - 0.15 mM的NaF也会导致cAMP产生显著增加。还发现NaF会改变分离的大鼠肝细胞中葡萄糖的产生。当将喂食大鼠的肝细胞与0.05 - 5 mM NaF一起孵育时，内源性来源释放的葡萄糖量增加。此外，NaF导致乳酸和丙酮酸产生减少。同样，NaF刺激禁食大鼠肝细胞中的葡萄糖产生。在约0.15 - 0.25 mM NaF时观察到最大刺激。当NaF浓度大于1.5 mM时，观察到葡萄糖产生减少。结论是NaF增加完整肝细胞中cAMP的水平并改变葡萄糖代谢。
