University of California, Irvine, Tu and Yuen Center for Functional Onco-Imaging, Irvine, California 92697, USA.
J Biomed Opt. 2011 Oct;16(10):106015. doi: 10.1117/1.3643342.
We present a magnetic resonance (MR)-guided near-infrared dynamic contrast enhanced diffuse optical tomography (DCE-DOT) system for characterization of tumors using an optical contrast agent (ICG) and a MR contrast agent [Gd-diethylenetriaminepentaacetic acid (DTPA)] in a rat model. Both ICG and Gd-DTPA are injected and monitored simultaneously using a combined MRI-DOT system, resulting in accurate co-registration between two imaging modalities. Fisher rats bearing R3230 breast tumor are imaged using this hybrid system. For the first time, enhancement kinetics of the exogenous contrast ICG is recovered from the DCE-DOT data using MR anatomical a priori information. As tumors grow, they undergo necrosis and the tissue transforms from viable to necrotic. The results show that the physiological changes between viable and necrotic tissue can be differentiated more accurately based on the ICG enhancement kinetics when MR anatomical information is utilized.
我们提出了一种磁共振（MR）引导的近红外动态对比增强漫射光学断层成像（DCE-DOT）系统，用于使用光学对比剂（ICG）和磁共振对比剂[钆-二乙三胺五乙酸（DTPA）]在大鼠模型中对肿瘤进行特征描述。ICG 和 Gd-DTPA 同时通过联合 MRI-DOT 系统进行注射和监测，从而在两种成像方式之间实现了准确的配准。使用该混合系统对携带 R3230 乳腺癌的 Fisher 大鼠进行成像。首次利用 MR 解剖先验信息从 DCE-DOT 数据中恢复出外源性对比剂 ICG 的增强动力学。随着肿瘤的生长，它们会发生坏死，组织从存活状态转变为坏死状态。结果表明，当利用 MR 解剖学信息时，基于 ICG 增强动力学可以更准确地区分存活组织和坏死组织的生理变化。
