[一种以单一标志物同时测定秦皮中四种香豆素的定量方法]

[Quantitative method for simultaneous assay of four coumarins with one marker in Fraxini Cortex].

作者信息

Feng Weihong, Wang Zhimin, Zhang Qiwei, Liu Limei, Wang Jinyu, Yang Fei

机构信息

Institute of Chinese Materia Madica, China Academy of Chinese Medical Sciences, Beijing 100700, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(13):1782-9.

PMID:22032145

Abstract

OBJECTIVE

To establish a new quantitative method for simultaneous determination of multi-coumarins in Fraxini Cortex by using one chemical reference substance, and validate its feasibilities.

METHOD

The new quality evaluation method, quantitative analysis of multi-components by singer-marker (QAMS), was established and validated with Fraxini Cortex. Four main coumarins were selected as analytes to evaluate the quality and their relative correlation factors (RCF) were determined by HPLC-DAD. Within the linear range, the values of RCF at 340 nm of aesculin to asculetin, fraxin and fraxetin were 1.771, 0.799, 1.409, respectively. And the contents of aesculin in samples of Fraxini Cortex were authentically determined by the external standard method, and the contents of the three other coumarins were calculated by their RCF. The contents of these four coumarins in all samples were also determined by the external standard method.

RESULT

Within a certain range, the RCF had a good reproducibility (RSD 2.5%-3.9%). Significant differences were not observed between the quantitative results of two methods.

CONCLUSION

It is feasible and suitable to evaluate the quality of Fraxini Cortex and its Yinpian by QAMS.

摘要

目的

采用单一化学对照品建立同时测定秦皮中多种香豆素的定量新方法，并验证其可行性。

方法

建立“一测多评法”（QAMS）这一新的质量评价方法，并用于秦皮的质量评价。选取4种主要香豆素作为分析对象，采用高效液相色谱-二极管阵列检测器（HPLC-DAD）法测定其相对校正因子（RCF）。在340 nm波长下，秦皮苷与秦皮素、秦皮甲素、秦皮乙素的RCF值分别为1.771、0.799、1.409。采用外标法测定秦皮中秦皮苷的含量，其余3种香豆素的含量根据其RCF计算得到。同时采用外标法测定所有样品中这4种香豆素的含量。