Weller M, Bresgen M, Heimann K, Wiedemann P
Department of Vitreoretinal Surgery, University Eye Clinic, Cologne, FRG.
Acta Ophthalmol (Copenh). 1990 Jun;68(3):246-52. doi: 10.1111/j.1755-3768.1990.tb01917.x.
The blood coagulation factor XIII catalyzes the crosslinking of fibrin monomers at the end of the coagulation cascade. Additional functions are the enzymatic coupling of fibrinectin to itself, fibrin, and collagen. We located the two subunits of factor XIII in 20 surgically obtained periretinal membranes, using double label immunofluorescence microscopy. Both subunits of factor XIII could be detected in all specimens. The positive staining in all specimens examined prompted us to determine the source of factor XIII. The abundant fibroblastic cells did not contain factor XIII. Macrophages, half of which stained for the alpha-subunit of factor XIII could not account for the presence of factor XIII because these cells were not present in all specimens, and did not stain for the beta-subunit. Factor XIII is probably derived from the exudation of plasma and platelets through disrupted blood-ocular barriers. This is confirmed by the detection of both subunits in vitreous aspirates from patients with proliferative intraocular disorders (n = 15) by Western blotting.
凝血因子 XIII 在凝血级联反应末期催化纤维蛋白单体的交联。其其他功能包括纤连蛋白自身、纤维蛋白和胶原蛋白之间的酶促偶联。我们使用双标记免疫荧光显微镜，在 20 个手术获取的视网膜前膜中定位了因子 XIII 的两个亚基。在所有标本中均能检测到因子 XIII 的两个亚基。所有检查标本中的阳性染色促使我们确定因子 XIII 的来源。丰富的成纤维细胞不含因子 XIII。巨噬细胞中，一半对因子 XIII 的α亚基染色，但由于并非所有标本中都存在这些细胞，且它们对β亚基不染色，所以无法解释因子 XIII 的存在。因子 XIII 可能源自血浆和血小板通过破损的血眼屏障渗出。这通过蛋白质印迹法在增殖性眼内疾病患者（n = 15）的玻璃体液抽吸物中检测到两个亚基得到证实。
