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多样脲原体对牛精子的侵袭

Invasion of Ureaplasma diversum in bovine spermatozoids.

作者信息

Buzinhani Melissa, Yamaguti Maurício, Oliveira Rosângela C, Cortez Beatriz A, Marques Lucas Miranda, Machado-Santelli Gláucia M, Assumpção Mayra Eo, Timenetsky Jorge

机构信息

Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brasil.

出版信息

BMC Res Notes. 2011 Oct 27;4:455. doi: 10.1186/1756-0500-4-455.

DOI:10.1186/1756-0500-4-455
PMID:22032232
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3219583/
Abstract

BACKGROUND

Ureaplasma diversum has been associated with infertility in cows. In bulls, this mollicute colonizes the prepuce and distal portion of the urethra and may infect sperm cells. The aim of this study is to analyze in vitro interaction of U. diversum isolates and ATCC strains with bovine spermatozoids. The interactions were observed by confocal microscopy and the gentamycin internalization assay.

FINDINGS

U. diversum were able to adhere to and invade spermatozoids after 30 min of infection. The gentamicin resistance assay confirmed the intracellularity and survival of U. diversum in bovine spermatozoids.

CONCLUSIONS

The intracellular nature of bovine ureaplasma identifies a new difficulty to control the reproductive of these animals.

摘要

背景

牛支原体与奶牛不育有关。在公牛中，这种柔膜菌定殖于包皮和尿道远端，可能感染精子细胞。本研究的目的是分析牛支原体分离株和美国典型培养物保藏中心（ATCC）菌株与牛精子的体外相互作用。通过共聚焦显微镜和庆大霉素内化试验观察相互作用。

研究结果

感染30分钟后，牛支原体能够黏附并侵入精子。庆大霉素抗性试验证实了牛支原体在牛精子中的细胞内生存情况。

结论

牛脲原体的细胞内特性为控制这些动物的繁殖带来了新的困难。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2bc/3219583/38dfdff6d940/1756-0500-4-455-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2bc/3219583/9f05a06e73cf/1756-0500-4-455-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2bc/3219583/a0c67e6dfec5/1756-0500-4-455-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2bc/3219583/38dfdff6d940/1756-0500-4-455-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2bc/3219583/9f05a06e73cf/1756-0500-4-455-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2bc/3219583/a0c67e6dfec5/1756-0500-4-455-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2bc/3219583/38dfdff6d940/1756-0500-4-455-3.jpg

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