Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo. Av. Professor Lineu Prestes, 1374. 05508-900, São Paulo, SP, Brazil.
BMC Microbiol. 2010 Mar 17;10:83. doi: 10.1186/1471-2180-10-83.
Understanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the Ureaplasma diversum mollicute, which is detected in healthy or diseased bovine. The invasion in Hep-2 cells of four clinical isolates and two reference strains of their ureaplasma was studied by Confocal Laser Scanning Microscopy and gentamicin invasion assay.
The isolates and strains used were detected inside the cells after infection of one minute without difference in the arrangement for adhesion and invasion. The adhesion was scattered throughout the cells, and after three hours, the invasion of the ureaplasmas surrounded the nuclear region but were not observed inside the nuclei. The gentamicin invasion assay detected that 1% of the ATCC strains were inside the infected Hep-2 cells in contrast to 10% to the clinical isolates. A high level of phospholipase C activity was also detected in all studied ureaplasma.
The results presented herein will help better understand U. diversum infections, aswell as cellular attachment and virulence.
由于多样性及其与宿主细胞的关系，理解柔膜体纲是具有挑战性的。入侵解释了与它们机会主义作用相关的问题。关于多形尿素支原体柔膜体纲的研究较少，该支原体在健康或患病的牛中均有检出。通过共聚焦激光扫描显微镜和庆大霉素入侵试验研究了 4 株临床分离株和 2 株参考株对 Hep-2 细胞的入侵。
感染 1 分钟后，未观察到黏附排列的差异，分离株和使用的菌株就已在细胞内被检测到。黏附物散在分布于细胞内，3 小时后，支原体的入侵包围了核区，但未观察到核内有入侵。庆大霉素入侵试验检测到，与临床分离株的 10%相比，ATCC 株系中有 1%在感染的 Hep-2 细胞内。还检测到所有研究的支原体均具有高水平的磷酸脂酶 C 活性。
本文的研究结果将有助于更好地理解 U. diversum 感染以及细胞黏附和毒力。
