INEB-Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.
Biomacromolecules. 2011 Dec 12;12(12):4183-95. doi: 10.1021/bm2008235. Epub 2011 Nov 8.
Chitosan (Ch) is a nontoxic and biocompatible polysaccharide extensively used in biomedical applications. Ch, as a polycation, can be combined with anionic polymers by layer-by-layer (LbL) self-assembly, giving rise to multilayered complexed architectures. These structures can be used in tissue engineering strategies, as drug delivery systems, or artificial matrices mimicking the extracellular microenvironment. In this work, Ch was combined with poly(γ-glutamic acid) (γ-PGA). γ-PGA is a polyanion, which was microbially produced, and is known for its low immunogenic reaction and low cytotoxicity. Multilayered ultrathin films were assembled by LbL, with a maximum of six layers. The interaction between both polymers was analyzed by: ellipsometry, quartz crystal microbalance with dissipation, Fourier transform infrared spectroscopy, atomic force microscopy, and zeta potential measurements. Ch/γ-PGA polyelectrolyte multilayers (PEMs) revealed no cytotoxicity according to ISO 10993-5. Overall, this study demonstrates that Ch can interact electrostatically with γ-PGA forming multilayered films. Furthermore, this study provides a comprehensive characterization of Ch/γ-PGA PEM structures, elucidating the contribution of each layer for the nanostructured films. These model surfaces can be useful substrates to study cell-biomaterial interactions in tissue regeneration.
壳聚糖（Ch）是一种无毒且生物相容的多糖，广泛应用于生物医学领域。Ch 作为一种聚阳离子，可以通过层层自组装（LbL）与阴离子聚合物结合，形成多层复合结构。这些结构可用于组织工程策略、作为药物传递系统或模拟细胞外微环境的人工基质。在这项工作中，壳聚糖（Ch）与聚（γ-谷氨酸）（γ-PGA）结合。γ-PGA 是一种聚阴离子，由微生物产生，因其免疫原性反应低和细胞毒性低而闻名。通过 LbL 组装了最多六层的多层超薄膜。通过椭圆测量法、石英晶体微天平耗散、傅里叶变换红外光谱、原子力显微镜和 Zeta 电位测量分析了两种聚合物之间的相互作用。根据 ISO 10993-5，Ch/γ-PGA 聚电解质多层膜（PEM）显示出无细胞毒性。总的来说，这项研究表明壳聚糖（Ch）可以与 γ-PGA 静电相互作用形成多层膜。此外，该研究还对 Ch/γ-PGA PEM 结构进行了全面表征，阐明了各层对纳米结构薄膜的贡献。这些模型表面可以作为研究组织再生中细胞-生物材料相互作用的有用基底。
