磺胺类药物对卡氏肺孢子虫二氢蝶酸合酶的抑制作用。
Inhibition of Pneumocystis carinii dihydropteroate synthetase by sulfa drugs.
作者信息
Merali S, Zhang Y, Sloan D, Meshnick S
机构信息
Department of Chemistry, City University of New York Medical School, City College of City University of New York, New York 10031.
出版信息
Antimicrob Agents Chemother. 1990 Jun;34(6):1075-8. doi: 10.1128/AAC.34.6.1075.
Abstract
A new reversed-phase high-pressure liquid chromatography assay procedure for dihydropteroate synthetase (DHPS) that involves the elution of the enzyme incubation solution with a series of three solvents of decreasing polarity (ammonium phosphate buffer, 10% methanol, and 50% methanol) was designed. By this procedure DHPS was detected in Escherichia coli and Pneumocystis carinii with specific activities of 450 and 14 U/mg, respectively. A comparison of the effects of five sulfa drugs on P. carinii DHPS activity revealed that dapsone is the most potent of these drugs.
摘要
设计了一种新的用于二氢蝶酸合酶（DHPS）的反相高效液相色谱分析方法，该方法涉及用一系列三种极性递减的溶剂（磷酸铵缓冲液、10%甲醇和50%甲醇）洗脱酶孵育溶液。通过该方法，在大肠杆菌和卡氏肺孢子虫中检测到了DHPS，其比活性分别为450和14 U/mg。比较了五种磺胺药物对卡氏肺孢子虫DHPS活性的影响，结果表明氨苯砜是这些药物中最有效的。
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