粉唑醇对酿酒酵母的体内效应及抗真菌特性分子基础的测定
In vivo effects of fenpropimorph on the yeast Saccharomyces cerevisiae and determination of the molecular basis of the antifungal property.
作者信息
Marcireau C, Guilloton M, Karst F
机构信息
Laboratoire de Biochimie et Génétique des Microorganismes, Université de Poitiers, France.
出版信息
Antimicrob Agents Chemother. 1990 Jun;34(6):989-93. doi: 10.1128/AAC.34.6.989.
Abstract
The effects of fenpropimorph on sterol biosynthesis and growth of Saccharomyces cerevisiae were examined to pinpoint the mode of action of fungicides that inhibit ergosterol biosynthesis. Taking advantage of sterol auxotrophy and sterol permeability in mutant strains, we show that growth inhibition is strongly correlated with inhibition of sterol biosynthesis. We confirm that in vivo and at low concentrations, fenpropimorph inhibits delta 8----delta 7-sterol isomerase, and in addition, when it is used at higher concentrations, it inhibits delta 14-sterol reductase. We show also that the fungistatic effect of fenpropimorph is not due to the accumulation of abnormal sterols in treated cells but is linked to the specific inhibition of ergosterol biosynthesis, leading to the arrest of cell proliferation in the unbudded G1 phase of the cell cycle.
摘要
研究了粉唑醇对酿酒酵母甾醇生物合成和生长的影响，以确定抑制麦角甾醇生物合成的杀菌剂的作用模式。利用突变菌株中的甾醇营养缺陷型和甾醇通透性，我们发现生长抑制与甾醇生物合成的抑制密切相关。我们证实，在体内和低浓度下，粉唑醇抑制δ8→δ7-甾醇异构酶，此外，当高浓度使用时，它抑制δ14-甾醇还原酶。我们还表明，粉唑醇的抑菌作用不是由于处理过的细胞中异常甾醇的积累，而是与麦角甾醇生物合成的特异性抑制有关，导致细胞周期中未出芽的G1期细胞增殖停滞。
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A requirement for ergosterol to permit growth of yeast sterol auxotrophs on cholestanol.酵母甾醇营养缺陷型在胆甾烷醇上生长需要麦角甾醇。
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