Dept. of Chemical Engineering, University of Almería, 04120 Almería, Spain.
Biotechnol Prog. 2012 Mar-Apr;28(2):467-73. doi: 10.1002/btpr.737. Epub 2011 Oct 27.
The commonly used shear protective agent Pluronic F68 (PF68) was toxic to the marine dinoflagellate microalga Protoceratium reticulatum, but had a shear-protective effect on it at concentrations of ≤ 0.5 g L(-1) . Supplementation of P. reticulatum cultures with PF68 actually increased the fluidity of the cell membrane; therefore, the shear protective effect of PF68 could not be ascribed to reduced membrane fluidity, an explanation that has been commonly used in relation to its shear protective effect on animal cells. Data are reported on the membrane fluidity of P. reticulatum and its response to the presence of PF68 under sublethal and lethal turbulence regimens. The membrane fluidity was found to depend strongly on the level of lipoperoxides in the cells produced under lethal agitation.
常用的剪切保护剂泊洛沙姆 F68（PF68）对海洋甲藻原甲藻具有毒性，但在浓度≤0.5 g L(-1) 时对其具有剪切保护作用。在 PF68 的存在下补充原甲藻培养物实际上增加了细胞膜的流动性；因此，PF68 的剪切保护作用不能归因于细胞膜流动性的降低，这一解释已在其对动物细胞的剪切保护作用中得到广泛应用。本文报道了原甲藻的膜流动性及其对亚致死和致死紊流条件下 PF68 存在的响应。结果表明，在致死搅拌下产生的细胞中，膜流动性强烈依赖于脂过氧化物的水平。
