Benstein Ruben Maximilian, Cebi Zehra, Podola Björn, Melkonian Michael
Botanisches Institut, Biozentrum Köln, Universität zu Köln, Lehrstuhl 1, Zülpicher Str. 47 b, 50674, Köln, Germany.
Mar Biotechnol (NY). 2014 Dec;16(6):621-8. doi: 10.1007/s10126-014-9581-0. Epub 2014 Jun 18.
Products from phototrophic dinoflagellates such as toxins or pigments are potentially important for applications in the biomedical sciences, especially in drug development. However, the technical cultivation of these organisms is often problematic due to their sensitivity to hydrodynamic (shear) stress that is a characteristic of suspension-based closed photobioreactors (PBRs). It is thus often thought that most species of dinoflagellates are non-cultivable at a technical scale. Recent advances in the development of biofilm PBRs that rely on immobilization of microalgae may hold potential to circumvent this major technical problem in dinoflagellate cultivation. In the present study, the dinoflagellate Symbiodinium voratum was grown immobilized on a Twin-Layer PBR for isolation of the carotenoid peridinin, an anti-cancerogenic compound. Biomass productivities ranged from 1.0 to 11.0 g m(-2) day(-1) dry matter per vertical growth surface and a maximal biomass yield of 114.5 g m(-2), depending on light intensity, supplementary CO2, and type of substrate (paper or polycarbonate membrane) used. Compared to a suspension culture, the performance of the Twin-Layer PBRs exhibited significantly higher growth rates and maximal biomass yield. In the Twin-Layer PBR a maximal peridinin productivity of 24 mg m(-2) day(-1) was determined at a light intensity of 74 μmol m(-2) s(-1), although the highest peridinin content per dry weight (1.7 % w/w) was attained at lower light intensities. The results demonstrate that a biofilm-based PBR that minimizes hydrodynamic shear forces is applicable to technical-scale cultivation of dinoflagellates and may foster biotechnological applications of these abundant marine protists.
光合自养型甲藻产生的产物，如毒素或色素，在生物医学科学应用中具有潜在的重要性，尤其是在药物开发方面。然而，由于这些生物体对流体动力（剪切）应力敏感，而这是基于悬浮的封闭式光生物反应器（PBR）的一个特性，因此其技术培养往往存在问题。所以人们常常认为大多数甲藻物种在技术规模上无法培养。依赖微藻固定化的生物膜PBR开发方面的最新进展，可能有潜力规避甲藻培养中的这一主要技术问题。在本研究中固定在双层PBR上培养了甲藻Symbiodinium voratum，以分离类胡萝卜素多甲藻素，一种抗癌化合物。根据光照强度、补充二氧化碳以及所用底物（纸或聚碳酸酯膜）的类型，垂直生长表面的生物量产率范围为1.0至11.0 g m(-2) 天(-1)干物质，最大生物量产量为114.5 g m(-2)。与悬浮培养相比，双层PBR的性能表现出显著更高的生长速率和最大生物量产量。在双层PBR中，光照强度为74 μmol m(-2) s(-1)时，多甲藻素的最大产率为24 mg m(-2) 天(-1)，尽管在较低光照强度下每干重的多甲藻素含量最高（1.7% w/w）。结果表明，一种能将流体动力剪切力降至最低的基于生物膜的PBR适用于甲藻的技术规模培养，并可能促进这些丰富的海洋原生生物的生物技术应用。
