López-Rosales L, García-Camacho F, Sánchez-Mirón A, Contreras-Gómez A, Molina-Grima E
Chemical Engineering Area, University of Almería, 04120 Almería, Spain.
Chemical Engineering Area, University of Almería, 04120 Almería, Spain.
Bioresour Technol. 2015 Dec;197:375-82. doi: 10.1016/j.biortech.2015.08.087. Epub 2015 Aug 29.
The dinoflagellate Karlodinium veneficum was grown in bubble column photobioreactors and a genetic algorithm-based stochastic search strategy used to find optimal values for the culture parameters gas flow rate, culture height, and nozzle sparger diameter. Cell production, concentration of reactive oxygen species (ROS), membrane fluidity and photosynthetic efficiency were studied throughout the culture period. Gas-flow rates below 0.26Lmin(-1), culture heights over 1.25m and a nozzle diameter of 1.5mm were found to provide the optimal conditions for cell growth, with an increase of 60% in cell production with respect to the control culture. Non-optimal conditions produced a sufficiently high shear stress to negatively affect cell growth and even produce cell death. Cell physiology was also severely affected in stressed cultures. The production of ROS increased by up to 200%, whereas cell membrane fluidity decreased by 60% relative to control cultures. Photosynthetic efficiency decreased concomitantly with membrane fluidity.
将有毒甲藻（Karlodinium veneficum）培养于鼓泡塔光生物反应器中，并采用基于遗传算法的随机搜索策略来寻找培养参数（气体流速、培养高度和喷嘴曝气器直径）的最佳值。在整个培养期间，对细胞产量、活性氧（ROS）浓度、膜流动性和光合效率进行了研究。结果发现，气体流速低于0.26Lmin(-1)、培养高度超过1.25m以及喷嘴直径为1.5mm时，可为细胞生长提供最佳条件，与对照培养相比，细胞产量增加了60%。非最佳条件会产生足够高的剪切应力，对细胞生长产生负面影响，甚至导致细胞死亡。在受胁迫的培养物中，细胞生理学也受到严重影响。与对照培养相比，ROS的产量增加了200%，而细胞膜流动性下降了60%。光合效率随膜流动性的降低而相应下降。
